Data file S1. Summary of RNA-seq, Mapping, and HTseq-count |
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adapted from
Akiyama-Oda and Oda, Sci. Adv. 6, eaba7261 (2020) |
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under a Creative Commons
Attribution License 4.0 (CC BY 4.0) |
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RNAi Gene |
Spider
ID |
Stage |
Egg Laying Day (after the first injection) |
RNA-seq |
Mapping |
HTseq-count |
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Row Reads |
After Trimming |
Mapped Reads |
After Filtering |
Counted Reads |
Ambiguous |
No feature |
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gfp |
KK598 |
Stage 3 |
Day -2 (Control) |
16,492,799 |
13,567,676 |
12,876,863 |
10,623,297 |
8,789,307 |
21,616 |
1,812,374 |
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gfp |
KK598 |
Stage 3 |
Day 13 |
16,985,671 |
12,895,250 |
12,139,343 |
9,739,954 |
7,966,180 |
19,194 |
1,754,580 |
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gfp |
KK598 |
Stage 3 |
Day 17 |
24,008,244 |
10,146,776 |
9,697,391 |
8,174,896 |
7,271,967 |
16,838 |
886,091 |
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gfp |
KK598 |
Stage 3 |
Day 22 |
16,874,617 |
12,278,010 |
11,339,101 |
9,189,402 |
7,770,761 |
20,227 |
1,398,414 |
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gfp |
KK598 |
Stage 5 |
Day -2 (Control) |
18,908,900 |
16,610,890 |
15,776,033 |
12,978,821 |
10,441,247 |
61,311 |
2,476,263 |
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gfp |
KK598 |
Stage 5 |
Day 13 |
19,479,674 |
14,826,687 |
14,162,983 |
11,856,894 |
9,956,683 |
46,623 |
1,853,588 |
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gfp |
KK598 |
Stage 5 |
Day 17 |
12,315,897 |
10,615,398 |
10,243,791 |
8,776,336 |
7,464,182 |
30,193 |
1,281,961 |
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gfp |
KK598 |
Stage 5 |
Day 22 |
17,205,373 |
15,668,990 |
14,996,300 |
12,551,004 |
10,543,487 |
59,399 |
1,948,118 |
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gfp |
T1412 |
Stage 3 |
Day -1 (Control) |
16,824,514 |
15,256,774 |
14,411,092 |
11,757,373 |
9,870,334 |
20,187 |
1,866,852 |
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gfp |
T1412 |
Stage 3 |
Day 15 |
18,649,565 |
14,622,537 |
13,711,756 |
10,931,573 |
8,808,495 |
21,192 |
2,101,886 |
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gfp |
T1412 |
Stage 3 |
Day 20 |
16,654,522 |
13,467,432 |
12,606,978 |
10,349,317 |
8,597,964 |
18,583 |
1,732,770 |
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gfp |
T1412 |
Stage 5 |
Day -1 (Control) |
18,631,166 |
17,919,370 |
17,593,463 |
14,617,705 |
12,467,917 |
46,383 |
2,103,405 |
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gfp |
T1412 |
Stage 5 |
Day 15 |
17,196,124 |
13,681,262 |
13,166,148 |
11,027,950 |
9,151,737 |
42,940 |
1,833,273 |
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gfp |
T1412 |
Stage 5 |
Day 20 |
17,949,638 |
16,306,575 |
15,698,755 |
13,271,784 |
11,240,321 |
43,327 |
1,988,136 |
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gfp |
NN749 |
Stage 3 |
Day -1 (Control) |
15,859,780 |
13,734,529 |
13,199,817 |
11,025,246 |
8,480,597 |
22,364 |
2,522,285 |
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gfp |
NN749 |
Stage 3 |
Day 21 |
19,015,317 |
14,367,046 |
13,724,392 |
11,542,995 |
9,444,013 |
24,096 |
2,074,886 |
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gfp |
NN749 |
Stage 5 |
Day -1 (Control) |
15,122,812 |
14,100,269 |
13,415,044 |
11,297,649 |
8,917,602 |
51,679 |
2,328,368 |
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gfp |
NN749 |
Stage 5 |
Day 21 |
15,475,341 |
14,236,345 |
13,491,362 |
11,270,495 |
8,965,391 |
49,674 |
2,255,430 |
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gfp |
27O |
Stage 5 |
Day -3 (Control) |
17,774,015 |
16,904,291 |
16,365,335 |
13,775,800 |
11,470,664 |
47,951 |
2,257,185 |
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gfp |
27O |
Stage 5 |
Day 19 |
16,156,752 |
14,942,383 |
14,384,298 |
12,157,403 |
9,839,024 |
47,875 |
2,270,504 |
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gfp |
27O |
Stage 6 |
Day -3 (Control) |
17,499,321 |
16,764,561 |
16,175,871 |
13,502,371 |
11,150,513 |
57,991 |
2,293,867 |
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gfp |
27O |
Stage 6 |
Day 19 |
17,443,451 |
16,961,715 |
16,331,853 |
13,664,774 |
11,302,611 |
66,552 |
2,295,611 |
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gfp |
27W |
Stage 5 |
Day -2 (Control) |
17,163,164 |
16,723,604 |
16,023,332 |
13,080,886 |
10,643,435 |
53,541 |
2,383,910 |
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gfp |
27W |
Stage 5 |
Day 20 |
17,499,411 |
16,872,009 |
16,062,129 |
13,024,595 |
10,325,438 |
46,605 |
2,652,552 |
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gfp |
27W |
Stage 6 |
Day -2 (Control) |
18,070,733 |
17,156,237 |
16,460,689 |
13,507,156 |
11,036,780 |
45,971 |
2,424,405 |
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gfp |
27W |
Stage 6 |
Day 20 |
16,893,997 |
16,295,455 |
15,505,478 |
12,539,103 |
10,105,237 |
46,081 |
2,387,785 |
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Pt-hh |
NN588 |
Stage 3 |
Day -2 (Control) |
16,069,834 |
15,826,127 |
15,163,107 |
12,143,810 |
8,603,928 |
16,753 |
3,523,129 |
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Pt-hh |
NN588 |
Stage 3 |
Day 16 |
12,486,855 |
12,363,753 |
11,825,435 |
9,533,242 |
6,924,595 |
13,866 |
2,594,781 |
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Pt-hh |
NN588 |
Stage 3 |
Day 20 |
18,065,485 |
17,803,617 |
17,112,045 |
13,717,380 |
9,738,213 |
20,765 |
3,958,402 |
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Pt-hh |
NN588 |
Stage 5 |
Day -2 (Control) |
13,301,712 |
13,204,651 |
12,615,778 |
10,279,411 |
7,119,180 |
22,681 |
3,137,550 |
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Pt-hh |
NN588 |
Stage 5 |
Day 16 |
12,913,063 |
12,856,505 |
12,391,172 |
10,156,581 |
7,369,918 |
22,507 |
2,764,156 |
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Pt-hh |
NN588 |
Stage 5 |
Day 20 |
11,996,819 |
11,948,396 |
11,388,758 |
9,219,333 |
6,188,457 |
15,569 |
3,015,307 |
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Pt-hh |
T1270 |
Stage 3 |
Day -2 (Control) |
11,898,582 |
11,848,138 |
11,007,826 |
8,696,751 |
5,961,730 |
10,498 |
2,724,523 |
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Pt-hh |
T1270 |
Stage 3 |
Day 18 |
14,215,950 |
14,100,190 |
13,263,331 |
10,282,261 |
6,856,904 |
13,402 |
3,411,955 |
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Pt-hh |
T1270 |
Stage 3 |
Day 22 |
17,212,315 |
17,051,432 |
16,067,597 |
12,951,311 |
9,473,776 |
19,403 |
3,458,132 |
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Pt-hh |
T1270 |
Stage 5 |
Day -2 (Control) |
14,567,673 |
14,486,231 |
13,843,737 |
11,172,879 |
7,528,185 |
28,505 |
3,616,189 |
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Pt-hh |
T1270 |
Stage 5 |
Day 18 |
14,247,298 |
14,183,586 |
13,563,817 |
11,064,687 |
7,855,054 |
28,388 |
3,181,245 |
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Pt-hh |
T1270 |
Stage 5 |
Day 22 |
16,818,562 |
16,757,547 |
15,877,285 |
12,954,195 |
8,992,530 |
32,342 |
3,929,323 |
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Pt-ptc |
23P |
Stage 3 |
Day -3 (Control) |
14,708,547 |
14,540,564 |
13,605,766 |
10,402,047 |
8,223,159 |
19,598 |
2,159,290 |
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Pt-ptc |
23P |
Stage 3 |
Day 14 |
16,534,644 |
16,245,650 |
15,606,585 |
12,765,721 |
10,582,480 |
25,632 |
2,157,609 |
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Pt-ptc |
23P |
Stage 3 |
Day 18 |
19,545,516 |
19,411,098 |
18,388,855 |
14,507,242 |
11,859,986 |
29,158 |
2,618,098 |
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Pt-ptc |
23P |
Stage 5 |
Day -3 (Control) |
16,745,773 |
16,658,864 |
16,002,283 |
12,855,542 |
10,518,807 |
61,318 |
2,275,417 |
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Pt-ptc |
23P |
Stage 5 |
Day 14 |
15,422,817 |
15,354,375 |
14,995,527 |
12,799,569 |
11,072,670 |
63,850 |
1,663,049 |
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Pt-ptc |
23P |
Stage 5 |
Day 18 |
16,063,637 |
16,007,823 |
15,476,180 |
12,735,680 |
10,788,056 |
65,628 |
1,881,996 |
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Pt-ptc |
23Z |
Stage 3 |
Day -1 (Control) |
17,972,232 |
16,705,348 |
15,732,147 |
12,631,803 |
10,784,923 |
25,795 |
1,821,085 |
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Pt-ptc |
23Z |
Stage 3 |
Day 14 |
25,590,154 |
25,168,388 |
24,038,580 |
19,707,457 |
16,400,327 |
41,353 |
3,265,777 |
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Pt-ptc |
23Z |
Stage 3 |
Day 19 |
15,894,934 |
14,142,265 |
13,878,243 |
11,358,449 |
9,236,576 |
24,156 |
2,097,717 |
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Pt-ptc |
23Z |
Stage 5 |
Day -1 (Control) |
19,043,994 |
16,401,551 |
16,079,515 |
13,595,812 |
11,479,868 |
68,409 |
2,047,535 |
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Pt-ptc |
23Z |
Stage 5 |
Day 14 |
22,865,750 |
22,566,577 |
22,032,396 |
18,454,042 |
15,212,108 |
123,594 |
3,118,340 |
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Pt-ptc |
23Z |
Stage 5 |
Day 19 |
20,271,357 |
18,481,001 |
17,190,926 |
14,549,885 |
12,356,742 |
87,862 |
2,105,281 |
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Pt-ptc |
24W |
Stage 3 |
Day -1 (Control) |
14,255,891 |
11,008,466 |
10,510,770 |
8,631,458 |
7,013,796 |
15,934 |
1,601,728 |
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Pt-ptc |
24W |
Stage 3 |
Day 14 |
13,103,424 |
10,424,448 |
9,932,186 |
8,189,676 |
6,918,468 |
15,298 |
1,255,910 |
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Pt-ptc |
24W |
Stage 3 |
Day 19 |
19,455,742 |
13,943,364 |
13,194,937 |
10,811,164 |
8,685,353 |
19,823 |
2,105,988 |
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Pt-ptc |
24W |
Stage 5 |
Day -1 (Control) |
18,602,936 |
17,569,706 |
15,720,552 |
14,232,710 |
11,620,400 |
59,572 |
2,552,738 |
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Pt-ptc |
24W |
Stage 5 |
Day 14 |
17,192,433 |
15,636,587 |
15,072,997 |
12,696,449 |
10,729,789 |
51,318 |
1,915,342 |
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Pt-ptc |
24W |
Stage 5 |
Day 19 |
16,472,642 |
14,241,093 |
13,720,908 |
11,519,213 |
9,405,490 |
48,821 |
2,064,902 |
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Pt-msx1 |
26LL |
Stage 5 |
Day -3 (Control) |
19,363,166 |
18,051,324 |
17,157,166 |
14,484,843 |
11,620,813 |
61,989 |
2,802,041 |
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Pt-msx1 |
26LL |
Stage 5 |
Day 18 |
18,722,381 |
17,125,664 |
16,164,891 |
13,540,125 |
10,362,530 |
78,358 |
3,099,237 |
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Pt-msx1 |
26LL |
Stage 6 |
Day -3 (Control) |
18,028,920 |
17,070,764 |
16,569,750 |
14,233,292 |
11,621,286 |
70,232 |
2,541,774 |
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Pt-msx1 |
26LL |
Stage 6 |
Day 18 |
18,350,258 |
16,540,120 |
15,878,650 |
13,455,332 |
10,670,229 |
71,165 |
2,713,938 |
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Pt-msx1 |
27j |
Stage 5 |
Day -3 (Control) |
17,409,072 |
16,980,127 |
16,305,169 |
13,629,278 |
11,263,991 |
59,586 |
2,305,701 |
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Pt-msx1 |
27j |
Stage 5 |
Day 14 |
18,134,173 |
14,214,267 |
13,737,625 |
11,619,919 |
9,940,041 |
43,860 |
1,636,018 |
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Pt-msx1 |
27j |
Stage 6 |
Day -3 (Control) |
16,847,922 |
16,193,507 |
15,631,425 |
13,184,636 |
11,063,289 |
64,947 |
2,056,400 |
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Pt-msx1 |
27j |
Stage 6 |
Day 14 |
16,065,994 |
14,834,082 |
14,351,153 |
12,172,199 |
10,225,628 |
53,040 |
1,893,531 |
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Pt-msx1 |
27t |
Stage 5 |
Day -3 (Control) |
15,246,253 |
14,549,974 |
14,066,153 |
10,865,120 |
9,106,833 |
61,436 |
1,696,851 |
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Pt-msx1 |
27t |
Stage 5 |
Day 24 |
19,102,690 |
16,685,655 |
16,047,692 |
12,304,069 |
10,230,057 |
76,338 |
1,997,674 |
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Pt-msx1 |
27t |
Stage 6 |
Day -3 (Control) |
15,189,780 |
14,522,002 |
13,999,379 |
10,686,815 |
8,725,236 |
66,902 |
1,894,677 |
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Pt-msx1 |
27t |
Stage 6 |
Day 24 |
19,306,496 |
15,135,359 |
14,569,101 |
11,530,905 |
9,697,445 |
62,488 |
1,770,972 |
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