RelatedData1.1 Cluster 0 markers identified in single-nucleus RNA-seq analysis of P. tepidariorum embryos at late stage 5

Gene ID (NCBI)

p_val

avg_log2FC

*pct.1

*pct.2

p_val_adj

Gene ID (NCBI)

Product (NCBI)

aug3 gene model

Expression in RNAi embryos**

LOC107445228

2.68280432

0.791

0.117

LOC107445228

protein gooseberry

aug3.g18397

ptc5 -

msx6 -

LOC107447180

2.4475954

0.926

0.276

LOC107447180

protein Wnt-5b-like

aug3.g1632

ptc5 -

LOC107450899

2.28697232

0.959

0.516

LOC107450899

sal-like protein 1

aug3.g3675

ptc5 -

msx6 -

fuchi5 -

LOC107451685

2.19231833

0.945

0.456

LOC107451685

roundabout homolog 3

aug3.g10604

hh5 +

ptc5 -

LOC107452006

1.99300229

0.872

0.31

LOC107452006

transcription factor AP-2-beta

aug3.g23531

hh5 +

ptc5 -

LOC107436465

1.87E-303

1.5496737

0.503

0.045

3.79E-299

LOC107436465

B-cell lymphoma/leukemia 11A

aug3.g26043

hh5 +

ptc5 -

LOC107443647

5.15E-219

1.6909794

0.837

0.4

1.05E-214

LOC107443647

plexin-B

aug3.g17369

fuchi5 -

LOC107438015

1.21E-210

0.97500173

0.391

0.042

2.46E-206

LOC107438015

growth arrest-specific protein 1

aug3.g9575

hh5 +

ptc5 -

msx6 -

LOC107447504

1.45E-192

1.36225618

0.549

0.125

2.95E-188

LOC107447504

homeobox protein Hox-B5b

aug3.g1787,aug3.g1786

ptc5 -

LOC107441660

4.42E-191

1.74038023

0.899

0.626

8.98E-187

LOC107441660

unconventional myosin-Ie

aug3.g9845,aug3.g9846,au

LOC107447678

3.87E-190

1.16299096

0.48

0.092

7.86E-186

LOC107447678

homeobox protein MSH-D

aug3.g15189

hh5 +

ptc5 -

LOC107440074

4.04E-184

1.96592468

0.342

0.038

8.20E-180

LOC107440074

uncharacterized LOC107440074

aug3.g407

hh5 +

ptc5 -

msx6 -

LOC107455285

1.65E-173

1.450861

0.457

0.101

3.35E-169

LOC107455285

abhydrolase domain-containing protein C22H12.03

aug3.g6972

ptc5 -

LOC107446080

5.09E-173

1.4125752

0.828

0.443

1.04E-168

LOC107446080

cyclin-dependent kinase 20

aug3.g1238

hh5 +

LOC107450670

8.58E-167

0.7998066

0.217

0.004

1.74E-162

LOC107450670

uncharacterized LOC107450670

aug3.g15599

ptc5 -

msx6 -

LOC107444844

1.51E-164

1.56341713

0.408

0.078

3.06E-160

LOC107444844

protein giant-lens

aug3.g839

ptc5 -

fuchi5 -

LOC107451660

3.08E-155

1.50115565

0.273

0.025

6.26E-151

LOC107451660

vitamin K-dependent protein C

aug3.g4198

ptc5 -

msx6 -

LOC107447499

1.17E-151

1.24552271

0.221

0.01

2.37E-147

LOC107447499

uncharacterized LOC107447499

LOC107444253

4.33E-148

1.25247369

0.504

0.142

8.80E-144

LOC107444253

transcription factor HES-1-A

aug3.g17723

ptc5 -

LOC107453612

4.29E-139

0.7506839

0.629

0.203

8.72E-135

LOC107453612

protein Wnt-8b-like

aug3.g5617

ptc5 -

fuchi5 -

LOC107446115

1.19E-135

1.01573723

0.894

0.64

2.41E-131

LOC107446115

extracellular sulfatase Sulf-1

aug3.g18939

ptc5 -

fuchi5 +

LOC107451332

2.56E-126

1.60339388

0.446

0.13

5.21E-122

LOC107451332

uncharacterized LOC107451332

aug3.g3963,aug3.g15729

ptc5 -

msx6 +

LOC107456525

8.98E-122

1.399866

0.563

0.216

1.82E-117

LOC107456525

delta-like protein 1

aug3.g25248

fuchi3 -

fuchi5 -

LOC107446595

4.66E-121

1.08407531

0.499

0.162

9.47E-117

LOC107446595

uncharacterized LOC107446595

aug3.g1396

ptc5 -

LOC107447970

2.87E-111

0.8024698

0.959

0.877

5.83E-107

LOC107447970

aurora kinase C

aug3.g15230

hh5 +

LOC107443364

2.60E-104

0.92395134

0.822

0.51

5.29E-100

LOC107443364

uncharacterized LOC107443364

aug3.g17172

hh5 +

LOC107444955

9.32E-100

1.56121749

0.528

0.246

1.89E-95

LOC107444955

uncharacterized LOC107444955

aug3.g887,aug3.g886

fuchi3 -

LOC107438301

9.91E-86

1.23827494

0.573

0.298

2.01E-81

LOC107438301

zinc finger protein 236

aug3.g12961

hh5 +

ptc5 -

LOC107454244

4.80E-84

0.60792982

0.989

0.945

9.76E-80

LOC107454244

axin-1

aug3.g6117

LOC107452883

4.97E-77

0.50624692

0.125

0.008

1.01E-72

LOC107452883

achaete-scute homolog 1

aug3.g5047

ptc5 -

msx6 -

LOC107447307

1.73E-76

0.80880421

0.596

0.291

3.51E-72

LOC107447307

uncharacterized LOC107447307

aug3.g21167

LOC107442050

2.40E-76

0.88488506

0.813

0.593

4.88E-72

LOC107442050

laminin subunit alpha-1

aug3.g16317

hh5 -

ptc5 -

LOC107456960

6.27E-71

0.73673862

0.872

0.698

1.27E-66

LOC107456960

suppressor of cytokine signaling 2

aug3.g25340,aug3.g25341

hh5 -

LOC107450335

3.11E-70

0.81389019

0.504

0.244

6.31E-66

LOC107450335

zinc finger protein basonuclin-2

aug3.g3273

hh5 +

ptc5 -

LOC107456506

4.79E-68

1.3766123

0.411

0.181

9.73E-64

LOC107456506

uncharacterized LOC107456506

aug3.g8629

hh5 +

LOC107442497

4.33E-66

0.77452755

0.136

0.016

8.80E-62

LOC107442497

uncharacterized LOC107442497

aug3.g27670,aug3.g20943

hh5 +

ptc5 -

msx6 -

fuchi3 -

LOC107439672

1.23E-63

0.69378941

0.669

0.424

2.50E-59

LOC107439672

serine/threonine-protein kinase pim-3

aug3.g13957

hh5 -

LOC107436894

2.03E-56

0.67349196

0.856

0.678

4.13E-52

LOC107436894

zinc finger protein rotund

aug3.g394

LOC107456521

6.55E-55

0.69225411

0.837

0.638

1.33E-50

LOC107456521

ephrin-B2a

aug3.g25262

ptc5 -

LOC107440619

8.52E-54

0.65410309

0.887

0.735

1.73E-49

LOC107440619

ETS domain-containing protein Elk-1

aug3.g9766

LOC107457368

1.52E-51

0.37701012

0.17

0.039

3.09E-47

LOC107457368

uncharacterized LOC107457368

aug3.g25891

LOC107442485

2.45E-51

0.72753157

0.704

0.489

4.98E-47

LOC107442485

partitioning defective 3 homolog

aug3.g27669

LOC107446220

9.44E-47

0.5404094

0.639

0.408

1.92E-42

LOC107446220

transcription factor Sox-14-like

aug3.g24914,aug3.g24896

hh5 +

LOC107451464

1.04E-46

0.58895528

0.591

0.373

2.11E-42

LOC107451464

transcription factor HES-4-A-like

aug3.g4026

ptc5 -

LOC107454821

6.00E-44

0.47551804

0.271

0.104

1.22E-39

LOC107454821

uncharacterized LOC107454821

aug3.g23640

LOC107441688

1.25E-43

0.55506258

0.216

0.072

2.53E-39

LOC107441688

recombining binding protein suppressor of hairless

aug3.g27389

LOC107443537

5.65E-43

0.43104333

0.99

0.93

1.15E-38

LOC107443537

uncharacterized LOC107443537

aug3.g471

LOC107440707

3.24E-41

0.77085887

0.262

0.105

6.58E-37

LOC107440707

homeobox protein Mix.2-like

aug3.g27124

ptc5 -

LOC107450465

4.11E-39

0.51281676

0.468

0.278

8.35E-35

LOC107450465

recombining binding protein suppressor of hairless

aug3.g15584

LOC107447872

1.59E-35

0.32441915

0.168

0.052

3.23E-31

LOC107447872

uncharacterized LOC107447872

aug3.g1960

ptc5 -

LOC107447507

3.03E-35

0.28918648

0.143

0.039

6.15E-31

LOC107447507

homeobox protein Hox-A5

aug3.g17640

hh5 +

ptc5 -

LOC107446454

1.04E-34

0.59867022

0.158

0.048

2.11E-30

LOC107446454

uncharacterized LOC107446454

aug3.g19176

LOC107443783

6.50E-33

0.4391563

0.299

0.145

1.32E-28

LOC107443783

plexin-A2

aug3.g428

LOC110283330

5.87E-31

0.45976602

0.336

0.177

1.19E-26

LOC110283330

uncharacterized LOC110283330

aug3.g21167,aug3.g11138

LOC107454926

3.47E-30

0.59470198

0.496

0.328

7.06E-26

LOC107454926

bcl-2-like protein 1

aug3.g23010,aug3.g6695,a

hh5 -

LOC107447651

3.49E-30

0.50018054

0.192

0.075

7.08E-26

LOC107447651

uncharacterized LOC107447651

aug3.g1855,aug3.g1854

LOC110281924

4.79E-30

0.53108342

0.65

0.489

9.73E-26

LOC110281924

triadin

aug3.g6466,aug3.g6465

hh5 -

ptc5 -

fuchi5 +

LOC107438714

8.26E-30

0.44436019

0.887

0.765

1.68E-25

LOC107438714

transcriptional activator cubitus interruptus-like

aug3.g13321

hh5 +

ptc5 -

LOC107441406

9.13E-29

0.6706598

0.565

0.407

1.85E-24

LOC107441406

uncharacterized LOC107441406

aug3.g27275

hh5 +

LOC107448079

1.13E-28

0.68673151

0.368

0.217

2.29E-24

LOC107448079

putative epidermal cell surface receptor

aug3.g20118

LOC107457295

5.41E-28

0.32599743

0.542

0.361

1.10E-23

LOC107457295

fructose-bisphosphate aldolase

aug3.g11046

LOC107457517

1.38E-27

0.49534321

0.742

0.59

2.80E-23

LOC107457517

G1/S-specific cyclin-D2

aug3.g11246,aug3.g11247

hh5 -

ptc5 -

LOC107454259

2.32E-27

0.37485204

0.256

0.126

4.70E-23

LOC107454259

protocadherin-like wing polarity protein stan

aug3.g15160,aug3.g6142,a

ptc5 -

LOC107448951

2.98E-27

0.48234459

0.173

0.068

6.06E-23

LOC107448951

BTB/POZ domain-containing protein kctd15-like

aug3.g2480

hh5 +

fuchi5 +

LOC107438758

4.15E-27

0.36114271

0.958

0.871

8.44E-23

LOC107438758

epidermal growth factor receptor

aug3.g13345,aug3.g13344

LOC107442208

9.14E-27

0.6198462

0.427

0.261

1.86E-22

LOC107442208

protein shifted

aug3.g27568

hh5 +

ptc5 -

LOC107447162

1.26E-26

0.51663571

0.317

0.174

2.55E-22

LOC107447162

protein C-ets-1

aug3.g19580

hh5 -

fuchi3 +

LOC107437786

5.91E-26

0.36033953

0.192

0.081

1.20E-21

LOC107437786

uncharacterized LOC107437786

aug3.g12585

hh5 -

ptc5 -

LOC107439278

9.47E-26

0.52009739

0.767

0.685

1.93E-21

LOC107439278

uncharacterized LOC107439278

aug3.g13670

LOC107444558

4.70E-25

0.42282365

0.406

0.243

9.55E-21

LOC107444558

paired box protein Pax-5

aug3.g762

ptc5 -

LOC107452888

1.11E-24

0.42685004

0.889

0.82

2.25E-20

LOC107452888

uncharacterized LOC107452888

aug3.g5060

hh5 +

ptc5 -

LOC107451864

1.82E-24

0.39151068

0.22

0.101

3.70E-20

LOC107451864

gonadotropin-releasing hormone II receptor

aug3.g4360

LOC107447988

1.88E-24

0.34824393

0.237

0.113

3.82E-20

LOC107447988

homeobox protein engrailed-like ceh-16

aug3.g15243

ptc5 -

LOC107450518

2.35E-24

0.41094475

0.88

0.759

4.77E-20

LOC107450518

neurogenic locus Notch protein

aug3.g21218

fuchi3 -

LOC107448091

1.18E-23

0.45665076

0.731

0.592

2.41E-19

LOC107448091

rab11 family-interacting protein 4B

aug3.g2082,aug3.g22713

hh5 +

LOC107455560

2.15E-23

0.35105522

0.284

0.154

4.38E-19

LOC107455560

cell division control protein 42 homolog

aug3.g7849,aug3.g7850

LOC107440837

2.27E-23

0.45803746

0.626

0.489

4.62E-19

LOC107440837

glutamate synthase [NADH]

aug3.g14860,aug3.g14861

LOC107456104

8.92E-23

0.3934451

0.327

0.186

1.81E-18

LOC107456104

uncharacterized LOC107456104

aug3.g10934,aug3.g18321

ptc5 -

LOC107445428

5.36E-22

0.43898041

0.462

0.308

1.09E-17

LOC107445428

BMP and activin membrane-bound inhibitor homolog

aug3.g18541

LOC107448451

1.27E-21

0.32440977

0.172

0.075

2.57E-17

LOC107448451

band 4.1-like protein 4

aug3.g2228

ptc5 -

LOC107451223

1.88E-21

0.37810393

0.409

0.269

3.82E-17

LOC107451223

uncharacterized protein DDB_G0271670

aug3.g21432

LOC107446742

8.27E-21

0.29410453

0.104

0.034

1.68E-16

LOC107446742

gamma-interferon-inducible lysosomal thiol reductase

aug3.g25649

LOC107456088

1.06E-20

0.4738545

0.428

0.28

2.16E-16

LOC107456088

iroquois-class homeodomain protein IRX-6

aug3.g8295

hh5 -

ptc5 -

msx6 +

fuchi3 +

LOC107444504

1.57E-19

0.294312

0.118

0.043

3.18E-15

LOC107444504

uncharacterized LOC107444504

aug3.g10054

msx6 -

LOC107451222

9.39E-19

0.40619272

0.212

0.111

1.91E-14

LOC107451222

uncharacterized LOC107451222

aug3.g21431

LOC107451441

1.15E-16

0.38824679

0.493

0.366

2.35E-12

LOC107451441

uncharacterized LOC107451441

aug3.g4013

LOC107453601

1.69E-16

0.435892

0.718

0.648

3.44E-12

LOC107453601

protein transport protein Sec24A

aug3.g5615

LOC107448985

2.30E-16

0.31134232

0.432

0.307

4.66E-12

LOC107448985

mucin-12

aug3.g2518

hh5 +

LOC107456634

5.03E-15

0.33069946

0.896

0.841

1.02E-10

LOC107456634

RNA polymerase-associated protein CTR9 homolog

aug3.g8712,aug3.g23794

LOC107452613

7.16E-15

0.35845753

0.803

0.731

1.45E-10

LOC107452613

lysine-specific demethylase 3B

aug3.g292

LOC107453131

1.82E-14

0.27309599

0.104

0.043

3.69E-10

LOC107453131

alpha-tocopherol transfer protein-like

aug3.g5234

LOC107453182

1.94E-14

0.34363551

0.465

0.352

3.93E-10

LOC107453182

elongation of very long chain fatty acids protein 6

aug3.g5294

LOC107450753

2.59E-14

0.2685792

0.932

0.888

5.27E-10

LOC107450753

membrane-associated guanylate kinase, WW and PDZ

aug3.g3592

LOC107448298

3.19E-14

0.30557279

0.743

0.67

6.49E-10

LOC107448298

transcriptional enhancer factor TEF-1

aug3.g15304

LOC107448055

4.50E-14

0.50583763

0.436

0.319

9.14E-10

LOC107448055

pro-epidermal growth factor

aug3.g2047

fuchi3 -

fuchi5 -

LOC107439669

1.04E-12

0.25385382

0.857

0.794

2.12E-08

LOC107439669

histone H3-like centromeric protein cid

aug3.g13949,aug3.g13948

LOC107452024

1.73E-12

0.32575961

0.146

0.078

3.52E-08

LOC107452024

uncharacterized LOC107452024

aug3.g4456

LOC107437115

1.84E-12

0.31377878

0.739

0.645

3.73E-08

LOC107437115

one cut domain family member 2

aug3.g26177

LOC107440093

1.86E-12

0.27299697

0.555

0.438

3.78E-08

LOC107440093

protein zwilch homolog

aug3.g26977

LOC107446561

2.68E-12

0.32206494

0.239

0.151

5.44E-08

LOC107446561

small conductance calcium-activated potassium chann

aug3.g1380,aug3.g23104

LOC107457070

6.02E-12

0.31498369

0.514

0.417

1.22E-07

LOC107457070

AP-2 complex subunit alpha-2

aug3.g9061,aug3.g9060

LOC107450720

3.00E-11

0.25136613

0.334

0.236

6.09E-07

LOC107450720

enhancer of rudimentary homolog

aug3.g21287

LOC107456664

5.75E-11

0.41868219

0.405

0.319

1.17E-06

LOC107456664

uncharacterized LOC107456664

aug3.g8740

LOC107452768

6.79E-11

0.26992189

0.262

0.173

1.38E-06

LOC107452768

cytokine receptor

aug3.g4975

fuchi5 -

LOC107441771

1.66E-10

0.29345763

0.637

0.544

3.36E-06

LOC107441771

protein tiptop

aug3.g25516

fuchi3 +

LOC107438320

5.32E-10

0.29140144

0.122

0.065

1.08E-05

LOC107438320

uncharacterized LOC107438320

aug3.g12989

hh5 +

ptc5 -

LOC107451426

8.36E-10

0.2691563

0.182

0.113

1.70E-05

LOC107451426

uncharacterized LOC107451426

aug3.g15724

LOC107451054

4.58E-09

0.25491223

0.334

0.244

9.31E-05

LOC107451054

zinc finger protein 41

aug3.g15673

LOC107453362

1.08E-08

0.2723636

0.534

0.445

0.00021863

LOC107453362

uncharacterized LOC107453362

aug3.g23776

LOC107441844

1.08E-08

0.28377993

0.442

0.358

0.00022036

LOC107441844

uncharacterized LOC107441844

aug3.g17675

LOC107446606

2.83E-08

0.26241623

0.382

0.308

0.0005745

LOC107446606

importin subunit alpha-1

aug3.g19295

LOC107455850

4.25E-08

0.2563464

0.501

0.42

0.00086397

LOC107455850

transmembrane protein 178B

aug3.g8086,aug3.g8085

ptc5 -

LOC107449746

7.92E-08

0.29412936

0.22

0.153

0.00160998

LOC107449746

nuclear receptor subfamily 2 group F member 1-A

aug3.g2915

hh5 +

ptc5 +

LOC107445188

1.69E-07

0.3151871

0.189

0.128

0.00342723

LOC107445188

AMP deaminase 2

aug3.g963

LOC107440811

6.84E-07

0.2779954

0.296

0.226

0.01389683

LOC107440811

centrosomal protein of 290 kDa

aug3.g27157,aug3.g27156

LOC107441035

1.09E-06

0.28071671

0.218

0.157

0.02220978

LOC107441035

connectin

aug3.g27196,aug3.g27195

ptc5 -

fuchi5 +

LOC107454354

4.02E-06

0.33239865

0.303

0.242

0.08160335

LOC107454354

semaphorin-5A

aug3.g6222

hh5 -