RelatedData1.2 Cluster 1 markers identified in single-nucleus RNA-seq analysis of P. tepidariorum embryos at late stage 5
Gene ID (NCBI) p_val avg_log2FC *pct.1 *pct.2 p_val_adj Gene ID (NCBI) Product (NCBI) aug3 gene model Expression in RNAi embryos**
LOC107454167 3.90E-301 1.98494173 0.919 0.397 7.93E-297 LOC107454167 dorsal-ventral patterning protein Sog-like aug3.g23966   ptc5 -       fuchi3 +  
LOC107448645 2.58E-251 1.82171166 0.915 0.415 5.24E-247 LOC107448645 transcription factor Sp9 aug3.g2333 hh5 - ptc5 -          
LOC107438948 3.01E-230 1.71794526 0.696 0.207 6.12E-226 LOC107438948 rhomboid-related protein 2 aug3.g13482 hh5 - ptc5 -   msx6 +      
LOC107444558 1.05E-170 1.33052908 0.615 0.189 2.14E-166 LOC107444558 paired box protein Pax-5 aug3.g762   ptc5 -          
LOC107442208 5.32E-161 1.37359385 0.632 0.208 1.08E-156 LOC107442208 protein shifted aug3.g27568 hh5 + ptc5 -          
LOC107450100 3.22E-159 1.52378452 0.737 0.306 6.55E-155 LOC107450100 homeobox protein DLX-6-like aug3.g15531   ptc5 -          
LOC107442769 7.21E-146 0.93129291 0.994 0.896 1.46E-141 LOC107442769 neurogenic locus Notch protein aug3.g27737,aug3.g21218              
LOC107437911 2.17E-137 1.24734119 0.582 0.212 4.40E-133 LOC107437911 serine protease 27 aug3.g12644 hh5 + ptc5 -          
LOC107446842 1.57E-135 0.93517571 0.98 0.795 3.19E-131 LOC107446842 uncharacterized LOC107446842 aug3.g1487,aug3.g1486 hh5 - ptc5 -       fuchi3 +  
LOC107456088 7.12E-108 1.26951014 0.583 0.24 1.45E-103 LOC107456088 iroquois-class homeodomain protein IRX-6 aug3.g8295 hh5 - ptc5 -   msx6 +   fuchi3 +  
LOC122273574 1.01E-106 0.79111242 0.431 0.129 2.04E-102 LOC122273574 serine protease 27-like aug3.g12644 hh5 + ptc5 -          
LOC107442927 3.17E-102 0.65924088 0.988 0.871 6.45E-98 LOC107442927 uncharacterized LOC107442927 　 　 　 　 　 　 　 　
LOC107438714 5.20E-101 0.79166026 0.952 0.749 1.06E-96 LOC107438714 transcriptional activator cubitus interruptus-like aug3.g13321 hh5 + ptc5 -          
LOC107454760 1.29E-87 0.88829959 0.854 0.609 2.62E-83 LOC107454760 ankyrin repeat domain-containing protein 6 aug3.g24117              
LOC107451631 3.81E-71 0.71985587 0.407 0.148 7.73E-67 LOC107451631 protein odd-skipped-related 2 aug3.g21549,aug3.g17123 hh5 - ptc5 -   msx6 +      
LOC107436691 1.64E-70 0.67015251 0.917 0.663 3.33E-66 LOC107436691 uncharacterized LOC107436691 aug3.g11765 hh5 - ptc5 -          
LOC107457539 3.17E-68 0.70721281 0.861 0.612 6.45E-64 LOC107457539 zinc finger homeobox protein 3 aug3.g11268 hh5 -         fuchi3 +  
LOC107436350 3.39E-49 0.51856438 0.953 0.819 6.89E-45 LOC107436350 glypican-6 aug3.g11833,aug3.g11486 hh5 - ptc5 +          
LOC107441868 5.59E-49 0.54320116 0.429 0.208 1.14E-44 LOC107441868 uncharacterized LOC107441868 aug3.g27457,aug3.g10400   ptc5 -          
LOC107451211 1.16E-45 0.59263644 0.57 0.35 2.35E-41 LOC107451211 ephrin type-B receptor 2 aug3.g3879 hh5 - ptc5 -          
LOC107448055 6.88E-45 0.8340016 0.52 0.298 1.40E-40 LOC107448055 pro-epidermal growth factor aug3.g2047           fuchi3 - fuchi5 -
LOC107436358 1.57E-42 0.41302749 0.973 0.823 3.18E-38 LOC107436358 fasciclin-2 aug3.g11491              
LOC107451394 1.82E-42 0.58024152 0.461 0.245 3.69E-38 LOC107451394 frizzled-5 aug3.g4009 hh5 -            
LOC107444121 2.99E-41 0.41142269 0.23 0.08 6.08E-37 LOC107444121 uncharacterized LOC107444121 aug3.g17638 hh5 - ptc5 -          
LOC107450033 2.54E-40 0.49632843 0.692 0.485 5.16E-36 LOC107450033 protein pangolin, isoforms A/H/I/S aug3.g10490,aug3.g18357              
LOC107456864 3.28E-37 0.48472342 0.933 0.808 6.66E-33 LOC107456864 exonuclease GOR-like aug3.g18504,aug3.g1256              
LOC107449282 6.41E-32 0.28682704 0.998 0.99 1.30E-27 LOC107449282 uncharacterized LOC107449282 aug3.g20669 hh5 - ptc5 +          
LOC110281924 7.98E-29 0.53504659 0.657 0.489 1.62E-24 LOC110281924 triadin aug3.g6466,aug3.g6465 hh5 - ptc5 -         fuchi5 +
LOC107447504 1.17E-28 0.54016223 0.349 0.185 2.37E-24 LOC107447504 homeobox protein Hox-B5b aug3.g1787,aug3.g1786   ptc5 -          
LOC107449746 2.81E-27 0.58542389 0.277 0.138 5.71E-23 LOC107449746 nuclear receptor subfamily 2 group F member 1-A aug3.g2915 hh5 + ptc5 +          
LOC107450063 8.96E-26 0.41418026 0.817 0.688 1.82E-21 LOC107450063 homeobox protein Meis1 aug3.g3111 hh5 -            
LOC107450690 2.68E-25 0.42451844 0.726 0.557 5.45E-21 LOC107450690 uncharacterized LOC107450690 aug3.g25707 hh5 + ptc5 +          
LOC107441380 7.53E-25 0.2716794 0.136 0.046 1.53E-20 LOC107441380 frizzled-4 aug3.g9827 hh5 - ptc5 -          
LOC107446167 3.90E-23 0.41339262 0.21 0.098 7.93E-19 LOC107446167 A disintegrin and metalloproteinase with thrombospondin motifs 18 aug3.g10171,aug3.g10170,aug3.g21920              
LOC122270833 6.55E-23 0.40990999 0.313 0.174 1.33E-18 LOC122270833 uncharacterized LOC122270833 aug3.g27084,aug3.g27085,aug3.g12520 hh5 +            
LOC107439920 6.57E-23 0.56006239 0.609 0.471 1.34E-18 LOC107439920 LIM and calponin homology domains-containing protein 1 aug3.g14125              
LOC107453655 1.62E-20 0.2826148 0.971 0.893 3.30E-16 LOC107453655 RNA-binding protein MEX3B aug3.g5665              
LOC107437822 9.66E-20 0.35613834 0.798 0.691 1.96E-15 LOC107437822 sentrin-specific protease 6 aug3.g26588              
LOC107456830 1.71E-17 0.27291124 0.777 0.642 3.47E-13 LOC107456830 serine-rich adhesin for platelets aug3.g8856 hh5 - ptc5 +          
LOC107451579 3.65E-17 0.30492495 0.697 0.536 7.41E-13 LOC107451579 E3 ubiquitin-protein ligase Rnf220 aug3.g4121 hh5 - ptc5 -          
LOC107440619 4.38E-17 0.27773403 0.847 0.749 8.89E-13 LOC107440619 ETS domain-containing protein Elk-1 aug3.g9766              
LOC122272478 9.80E-17 0.28874688 0.416 0.285 1.99E-12 LOC122272478 uncharacterized LOC122272478 　 　 　 　 　 　 　 　
LOC107436894 1.33E-15 0.30661634 0.813 0.692 2.70E-11 LOC107436894 zinc finger protein rotund aug3.g394              
LOC107439046 2.14E-15 0.29904938 0.311 0.199 4.34E-11 LOC107439046 homeobox protein extradenticle aug3.g13538,aug3.g27748,aug3.g27747 hh5 -            
LOC107440707 6.40E-15 0.34654182 0.215 0.12 1.30E-10 LOC107440707 homeobox protein Mix.2-like aug3.g27124   ptc5 -          
LOC107455121 7.59E-15 0.40079701 0.512 0.393 1.54E-10 LOC107455121 tyrosine-protein phosphatase 99A aug3.g6819,aug3.g6820 hh5 -            
LOC107444194 1.38E-14 0.27026611 0.222 0.127 2.79E-10 LOC107444194 zinc finger protein 658B aug3.g9387,aug3.g27551              
LOC107440894 3.15E-14 0.31628418 0.441 0.322 6.39E-10 LOC107440894 latrophilin Cirl aug3.g56              
LOC107452032 7.78E-14 0.28983096 0.347 0.237 1.58E-09 LOC107452032 protein dead ringer homolog aug3.g4461              
LOC107445214 1.92E-13 0.35014731 0.316 0.209 3.90E-09 LOC107445214 sodium-dependent neutral amino acid transporter B(0)AT3 aug3.g18382,aug3.g18383              
LOC107438901 3.90E-13 0.36514723 0.463 0.353 7.93E-09 LOC107438901 uncharacterized LOC107438901 aug3.g13449,aug3.g19865,aug3.g19863,aug3.g19111,aug3.g15841,aug3.g21682,aug3.g22706,aug3.g10878,aug3.g17675,aug3.g17718,aug3.g16185,aug3.g14065,aug3.g10641,aug3.g17719,aug3.g12613,aug3.g27455 hh5 +         fuchi3 -  
LOC107451091 1.30E-12 0.25458327 0.116 0.054 2.65E-08 LOC107451091 uncharacterized LOC107451091 aug3.g3782,aug3.g11118 hh5 +            
LOC107441411 9.85E-12 0.39529543 0.46 0.362 2.00E-07 LOC107441411 uncharacterized LOC107441411 aug3.g9829              
LOC107442777 1.05E-11 0.28695685 0.69 0.609 2.13E-07 LOC107442777 U3 small nucleolar ribonucleoprotein protein IMP3 aug3.g27738              
LOC107444292 1.98E-11 0.3453041 0.511 0.409 4.02E-07 LOC107444292 3-hydroxy-3-methylglutaryl-coenzyme A reductase aug3.g10040,aug3.g10041 hh5 +            
LOC107440815 4.02E-11 0.27507836 0.427 0.32 8.16E-07 LOC107440815 uncharacterized LOC107440815 aug3.g27168              
LOC122270927 6.36E-11 0.3960201 0.323 0.228 1.29E-06 LOC122270927 uncharacterized LOC122270927 aug3.g142             fuchi5 +
LOC107447950 4.94E-09 0.34237539 0.334 0.253 0.00010039 LOC107447950 protein lifeguard 2 aug3.g15222,aug3.g6216 hh5 +            
LOC107455850 5.22E-09 0.28558139 0.518 0.417 1.06E-04 LOC107455850 transmembrane protein 178B aug3.g8086,aug3.g8085   ptc5 -          
LOC107452507 5.91E-08 0.27511177 0.583 0.505 1.20E-03 LOC107452507 vacuolar protein sorting-associated protein 13B aug3.g4797              
LOC107454516 7.97E-07 0.25743464 0.358 0.285 0.01619206 LOC107454516 uncharacterized LOC107454516 aug3.g6342 hh5 +