| Table S4.1. Cluster 1 markers identified in the first single-cell
RNA-seq analysis. |
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| Gene ID (NCBI) |
p_val |
avg_log2FC |
*pct.1 |
*pct.2 |
p_val_adj |
Gene ID (NCBI) |
Product (NCBI) |
aug3 gene model |
Gene name |
**Expression in RNAi embryos |
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| LOC107440133 |
2.29E-63 |
2.09806135 |
0.745 |
0.022 |
4.04E-59 |
LOC107440133 |
twist-related
protein 2-like |
aug3.g14287 |
Pt-twi |
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| LOC107449287 |
4.59E-51 |
1.61882412 |
0.596 |
0.015 |
8.11E-47 |
LOC107449287 |
transcription
factor Sox-14-like |
aug3.g2665 |
|
hh5 - |
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| LOC107451770 |
7.79E-47 |
1.46874668 |
0.702 |
0.044 |
1.38E-42 |
LOC107451770 |
uncharacterized
LOC107451770, transcript variant X1 |
aug3.g4286 |
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hh5 - |
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| LOC107437681 |
5.62E-45 |
2.66988766 |
0.936 |
0.138 |
9.93E-41 |
LOC107437681 |
integrin
alpha-8-like |
aug3.g7077 |
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hh5 - |
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| LOC107450281 |
1.71E-42 |
2.21902277 |
0.957 |
0.129 |
3.03E-38 |
LOC107450281 |
group XV
phospholipase A2 |
aug3.g15560 |
|
hh5 - |
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fuchi3 - |
fuchi5 - |
| LOC107436092 |
1.49E-38 |
1.28537527 |
0.638 |
0.044 |
2.62E-34 |
LOC107436092 |
LIM domain only
protein 3, transcript variant X3 |
aug3.g11301 |
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hh5 - |
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| LOC107455300 |
2.66E-37 |
1.63799259 |
0.936 |
0.151 |
4.69E-33 |
LOC107455300 |
silk gland
factor 1-like |
aug3.g6985 |
Pt-fkh |
hh5 - |
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fuchi5 - |
| LOC107437526 |
3.25E-31 |
1.25546403 |
0.745 |
0.105 |
5.75E-27 |
LOC107437526 |
fibroblast
growth factor receptor 3-like, transcript variant
X1 |
aug3.g26271 |
Pt-FGFR-a |
hh5 - |
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fuchi5 - |
| LOC107445669 |
6.24E-27 |
1.37945332 |
0.766 |
0.12 |
1.10E-22 |
LOC107445669 |
uncharacterized
LOC107445669 |
aug3.g1125 |
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fuchi3 - |
fuchi5 - |
| LOC107438841 |
5.22E-26 |
1.01185208 |
0.723 |
0.127 |
9.22E-22 |
LOC107438841 |
fibroblast
growth factor receptor 4 |
aug3.g26565 |
Pt-FGFR-b |
hh5 - |
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| LOC107437853 |
1.89E-25 |
0.87700769 |
0.468 |
0.039 |
3.34E-21 |
LOC107437853 |
uncharacterized
LOC107437853 |
aug3.g12633 |
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| LOC107455742 |
5.96E-24 |
1.41657759 |
0.851 |
0.249 |
1.05E-19 |
LOC107455742 |
protein
hunchback-like, transcript variant X2 |
aug3.g25126 |
Pt-hb |
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| LOC107457179 |
1.10E-22 |
1.76662597 |
0.957 |
0.45 |
1.94E-18 |
LOC107457179 |
CD109 antigen,
transcript variant X1 |
aug3.g9168 |
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| LOC107437962 |
5.63E-19 |
2.07623105 |
0.979 |
0.644 |
9.94E-15 |
LOC107437962 |
uncharacterized
LOC107437962 |
aug3.g9571 |
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| LOC107443771 |
1.11E-18 |
0.84399239 |
0.872 |
0.247 |
1.95E-14 |
LOC107443771 |
protein FAM107B,
transcript variant X2 |
aug3.g17447 |
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fuchi5 - |
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| LOC107436524 |
1.48E-18 |
0.91122237 |
0.681 |
0.146 |
2.61E-14 |
LOC107436524 |
amyloid beta A4
precursor protein-binding family B member
1-interacting protein, transcript variant X3 |
aug3.g11631 |
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fuchi5 - |
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| LOC107449884 |
5.70E-18 |
0.79686974 |
0.532 |
0.09 |
1.01E-13 |
LOC107449884 |
notch-regulated
ankyrin repeat-containing protein-like |
aug3.g15506 |
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ptc5 - |
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fuchi5 - |
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| LOC107441450 |
7.66E-18 |
0.5449618 |
0.34 |
0.031 |
1.35E-13 |
LOC107441450 |
proto-oncogene
tyrosine-protein kinase ROS |
aug3.g9836 |
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| LOC107451631 |
2.50E-15 |
0.57535305 |
0.383 |
0.052 |
4.42E-11 |
LOC107451631 |
protein
odd-skipped-related 2-like |
aug3.g21549 |
Pt-odd-skipped related-B (osr-B) |
hh5 - |
ptc5 - |
msx6 + |
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| LOC107448763 |
4.65E-15 |
0.89326149 |
0.553 |
0.133 |
8.22E-11 |
LOC107448763 |
SLIT-ROBO Rho
GTPase-activating protein 1 |
aug3.g2394 |
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| LOC107438411 |
6.78E-15 |
0.74573332 |
0.34 |
0.041 |
1.20E-10 |
LOC107438411 |
roundabout
homolog 1-like |
aug3.g9591 |
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| LOC107440147 |
7.58E-15 |
1.47660543 |
0.979 |
0.769 |
1.34E-10 |
LOC107440147 |
protein singed,
transcript variant X2 |
aug3.g26989 |
Pt-fascin |
hh5 - |
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| LOC107446990 |
8.88E-15 |
0.42382645 |
0.277 |
0.024 |
1.57E-10 |
LOC107446990 |
zinc finger
E-box-binding homeobox 2 |
aug3.g10715 |
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| LOC107449499 |
2.24E-13 |
0.42780729 |
0.255 |
0.024 |
3.96E-09 |
LOC107449499 |
rhoGEF
domain-containing protein gxcJ-like |
aug3.g15443 |
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fuchi3 - |
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| LOC107445228 |
2.45E-13 |
0.6840286 |
0.745 |
0.207 |
4.32E-09 |
LOC107445228 |
protein
gooseberry, transcript variant X2 |
aug3.g18397 |
Pt-prd2
(pax3/7B) |
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ptc5 - |
msx6 - |
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| LOC107450121 |
7.33E-13 |
0.80094188 |
0.553 |
0.142 |
1.29E-08 |
LOC107450121 |
CD9 antigen-like |
aug3.g10506 |
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| LOC107443540 |
8.17E-13 |
1.01183939 |
0.936 |
0.755 |
1.44E-08 |
LOC107443540 |
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| LOC107457308 |
1.07E-12 |
0.64075657 |
0.426 |
0.079 |
1.89E-08 |
LOC107457308 |
calcium-activated
chloride channel regulator 2 |
aug3.g11056 |
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fuchi3 - |
fuchi5 - |
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| LOC107455061 |
2.00E-12 |
0.80125028 |
0.532 |
0.14 |
3.54E-08 |
LOC107455061 |
myosin-I heavy
chain |
aug3.g337 |
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hh5 - |
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| LOC107447467 |
3.62E-12 |
0.37961462 |
0.277 |
0.033 |
6.39E-08 |
LOC107447467 |
putative ankyrin
repeat protein RF_0381 |
aug3.g1767 |
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fuchi3 - |
fuchi5 - |
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| LOC107442019 |
6.80E-12 |
1.00493128 |
0.936 |
0.581 |
1.20E-07 |
LOC107442019 |
microsomal
glutathione S-transferase 3-like |
aug3.g27509 |
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fuchi5 - |
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| LOC107436800 |
8.01E-12 |
0.96490471 |
0.681 |
0.245 |
1.41E-07 |
LOC107436800 |
uncharacterized
LOC107436800 |
aug3.g11835 |
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fuchi5 + |
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| LOC107438844 |
1.22E-11 |
0.75631175 |
0.489 |
0.114 |
2.15E-07 |
LOC107438844 |
tubulointerstitial
nephritis antigen-like, transcript variant X2 |
aug3.g26570 |
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| LOC107454170 |
1.68E-11 |
0.48005306 |
0.447 |
0.092 |
2.96E-07 |
LOC107454170 |
phosphatidylinositol
4,5-bisphosphate 3-kinase catalytic subunit delta
isoform |
aug3.g23964 |
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fuchi5 - |
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| LOC107455997 |
2.18E-11 |
0.83888888 |
0.681 |
0.253 |
3.84E-07 |
LOC107455997 |
putative
transcription factor SOX-14 |
aug3.g8208 |
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| LOC107436641 |
7.52E-11 |
0.59557874 |
0.383 |
0.081 |
1.33E-06 |
LOC107436641 |
disco-interacting
protein 2, transcript variant X1 |
aug3.g11718 |
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hh5 - |
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| LOC107456233 |
2.86E-10 |
0.83738418 |
0.979 |
0.834 |
5.05E-06 |
LOC107456233 |
beta-amyloid-like
protein |
aug3.g8414 |
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fuchi3 - |
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| LOC107457564 |
6.73E-10 |
0.61367281 |
0.319 |
0.061 |
1.19E-05 |
LOC107457564 |
homeobox protein
OTX2, transcript variant X1 |
aug3.g25936 |
Pt-otx2 |
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| LOC107443762 |
9.19E-10 |
0.52995571 |
0.383 |
0.087 |
1.62E-05 |
LOC107443762 |
dual specificity
protein phosphatase 7 |
aug3.g526 |
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msx6 + |
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| LOC107451344 |
6.92E-09 |
0.94172154 |
0.787 |
0.496 |
1.22E-04 |
LOC107451344 |
protein
C-ets-2-like |
aug3.g3964 |
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hh5 - |
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| LOC107441543 |
7.24E-09 |
0.60509257 |
0.851 |
0.52 |
1.28E-04 |
LOC107441543 |
neurotactin-like,
transcript variant X1 |
aug3.g27319 |
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hh5 - |
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fuchi5 - |
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| LOC107452121 |
7.38E-09 |
0.61144764 |
0.511 |
0.157 |
1.30E-04 |
LOC107452121 |
uncharacterized
LOC107452121, transcript variant X1 |
aug3.g4530 |
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| LOC107453693 |
8.61E-09 |
0.5682267 |
0.362 |
0.081 |
1.52E-04 |
LOC107453693 |
multiple
coagulation factor deficiency protein 2 homolog,
transcript variant X2 |
aug3.g12187 |
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| LOC107452100 |
9.66E-09 |
0.36659426 |
0.34 |
0.074 |
1.71E-04 |
LOC107452100 |
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| LOC107453313 |
9.72E-09 |
0.96510645 |
0.83 |
0.452 |
1.72E-04 |
LOC107453313 |
protein naked
cuticle homolog 1 |
aug3.g5391 |
Pt-naked cuticle |
hh5 - |
ptc5 + |
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| LOC107455216 |
1.47E-08 |
0.78584041 |
0.447 |
0.138 |
2.61E-04 |
LOC107455216 |
bestrophin-2 |
aug3.g6914 |
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hh5 + |
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| LOC107441706 |
3.46E-08 |
0.43676016 |
1 |
0.897 |
6.11E-04 |
LOC107441706 |
ribosome-binding
protein 1 |
aug3.g16056 |
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fuchi5 - |
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| LOC107450858 |
3.74E-08 |
0.44083415 |
0.319 |
0.074 |
6.60E-04 |
LOC107450858 |
uncharacterized
protein C11orf70 |
aug3.g3648 |
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| LOC107456902 |
3.88E-08 |
0.49998666 |
0.404 |
0.111 |
6.86E-04 |
LOC107456902 |
uncharacterized
LOC107456902, transcript variant X1 |
aug3.g5686 |
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| LOC107445091 |
5.31E-08 |
0.51198003 |
0.255 |
0.048 |
9.39E-04 |
LOC107445091 |
protein
scabrous-like |
aug3.g943 |
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fuchi5 - |
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| LOC107438705 |
1.55E-07 |
0.29971404 |
0.532 |
0.175 |
2.73E-03 |
LOC107438705 |
SEC14-like
protein 2 |
aug3.g13303 |
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fuchi3 - |
fuchi5 - |
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| LOC107453680 |
1.81E-07 |
0.6067549 |
0.447 |
0.151 |
3.21E-03 |
LOC107453680 |
myosin-11-like |
aug3.g23853 |
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| LOC107449707 |
2.40E-07 |
0.76647248 |
0.66 |
0.334 |
4.24E-03 |
LOC107449707 |
abnormal cell
migration protein 10-like, transcript variant X2 |
aug3.g2910 |
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| LOC107455066 |
2.72E-07 |
0.54540474 |
0.255 |
0.055 |
4.81E-03 |
LOC107455066 |
uncharacterized
LOC107455066, transcript variant X3 |
aug3.g336 |
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hh5 - |
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msx6 - |
fuchi3 - |
fuchi5 - |
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| LOC107442891 |
3.47E-07 |
0.37078546 |
0.277 |
0.061 |
6.13E-03 |
LOC107442891 |
zinc finger
protein ush, transcript variant X2 |
aug3.g16893 |
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| LOC107444665 |
4.06E-07 |
0.48764635 |
0.383 |
0.114 |
7.17E-03 |
LOC107444665 |
nuclear receptor
subfamily 2 group E member 1-like |
aug3.g794 |
Pt-tll |
hh5 - |
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fuchi5 - |
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| LOC110282220 |
4.53E-07 |
0.2694527 |
0.277 |
0.061 |
8.01E-03 |
LOC110282220 |
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| LOC107438548 |
5.53E-07 |
0.34305985 |
0.255 |
0.055 |
9.77E-03 |
LOC107438548 |
uncharacterized
LOC107438548, transcript variant X1 |
aug3.g13175 |
|
hh5 + |
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fuchi3 + |
fuchi5 + |
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| *pct1 and pct2
mean proportion of cells that express the gene in this cluster and other
clusters, respectively. |
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| **hh5, hh pRNAi at late stage 5;
ptc5. ptc
pRNAi at late stage 5; msx6, msx1 pRNAi at stage 6;
fuchi3, fuchi pRNAi at stage 3;
fuchi5, fuchi pRNAi at stage 5; +, expression increase; -, expression
decrease |
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