| Table S4.5. Cluster 5 markers identified in the first
single-cell RNA-seq analysis. |
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| Gene ID (NCBI) |
p_val |
avg_log2FC |
pct.1* |
pct.2* |
p_val_adj |
Gene ID (NCBI) |
Product (NCBI) |
aug3 gene model |
Gene name |
Expression in RNAi embryos** |
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| LOC107447180 |
2.27E-48 |
1.80040835 |
0.866 |
0.157 |
4.00E-44 |
LOC107447180 |
protein
Wnt-5b-like, transcript variant X1 |
aug3.g10820 |
Pt-wnt5 |
|
ptc5 - |
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| LOC107451660 |
3.92E-44 |
2.80818465 |
0.742 |
0.123 |
6.93E-40 |
LOC107451660 |
vitamin
K-dependent protein C-like |
aug3.g4198 |
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ptc5 - |
msx6 - |
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| LOC107445228 |
1.71E-35 |
1.52489052 |
0.722 |
0.147 |
3.01E-31 |
LOC107445228 |
protein
gooseberry, transcript variant X2 |
aug3.g18397 |
Pt-prd2 |
|
ptc5 - |
msx6 - |
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| LOC107444844 |
1.30E-31 |
1.66702738 |
0.649 |
0.13 |
2.29E-27 |
LOC107444844 |
protein
giant-lens-like |
aug3.g839 |
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ptc5 - |
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|
fuchi5 - |
| LOC107450899 |
3.00E-31 |
1.7394002 |
0.866 |
0.324 |
5.31E-27 |
LOC107450899 |
sal-like protein
1 |
aug3.g3675 |
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ptc5 - |
msx6 - |
|
fuchi5 - |
| LOC107451065 |
5.05E-31 |
1.13151783 |
0.412 |
0.025 |
8.92E-27 |
LOC107451065 |
uncharacterized
LOC107451065 |
aug3.g3755 |
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ptc5 - |
msx6 - |
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| LOC107447504 |
1.78E-30 |
1.22814522 |
0.68 |
0.159 |
3.14E-26 |
LOC107447504 |
homeobox protein
Hox-B4a-like, transcript variant X1 |
aug3.g1787 |
Pt-ftz |
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ptc5 - |
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| LOC107440074 |
1.81E-30 |
2.1964606 |
0.608 |
0.11 |
3.20E-26 |
LOC107440074 |
uncharacterized
LOC107440074 |
aug3.g407 |
Pt-RTP-B |
hh5 + |
ptc5 - |
msx6 - |
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| LOC107451685 |
7.04E-30 |
1.38643207 |
0.918 |
0.449 |
1.24E-25 |
LOC107451685 |
roundabout
homolog 3, transcript variant X4 |
aug3.g10604 |
Pt-echinoid |
hh5 + |
ptc5 - |
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| LOC107443364 |
1.55E-27 |
1.22439996 |
0.979 |
0.762 |
2.74E-23 |
LOC107443364 |
uncharacterized
LOC107443364, transcript variant X2 |
aug3.g17172 |
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hh5 + |
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| LOC107443647 |
1.39E-25 |
1.40165789 |
0.794 |
0.304 |
2.45E-21 |
LOC107443647 |
plexin-B |
aug3.g17369 |
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fuchi5 - |
| LOC107438015 |
1.14E-24 |
0.91033546 |
0.495 |
0.081 |
2.02E-20 |
LOC107438015 |
growth
arrest-specific protein 1 |
aug3.g9575 |
Pt-gas1 |
hh5 + |
ptc5 - |
msx6 - |
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| LOC107454244 |
3.61E-24 |
1.15215534 |
0.99 |
0.919 |
6.38E-20 |
LOC107454244 |
axin-2-like |
aug3.g6117 |
Pt-axin |
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| LOC107447678 |
2.16E-23 |
1.05934025 |
0.691 |
0.194 |
3.82E-19 |
LOC107447678 |
homeobox protein
MSH-D-like |
aug3.g15189 |
Pt-msx1 |
hh5 + |
ptc5 - |
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| LOC107452006 |
4.17E-20 |
0.63615695 |
0.412 |
0.066 |
7.36E-16 |
LOC107452006 |
transcription
factor AP-2-beta-like, transcript variant X2 |
aug3.g23531 |
Pt-AP2-A |
hh5 + |
ptc5 - |
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| LOC107441660 |
1.24E-18 |
0.899668 |
0.619 |
0.201 |
2.19E-14 |
LOC107441660 |
unconventional
myosin-Ie |
aug3.g22123 |
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| LOC107444955 |
3.00E-18 |
0.74599018 |
0.289 |
0.027 |
5.30E-14 |
LOC107444955 |
uncharacterized
LOC107444955, transcript variant X2 |
aug3.g886 |
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fuchi3 - |
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| LOC107446595 |
1.13E-17 |
1.02184559 |
0.66 |
0.252 |
1.99E-13 |
LOC107446595 |
uncharacterized
LOC107446595 |
aug3.g1396 |
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ptc5 - |
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| LOC107446454 |
1.02E-16 |
0.93946052 |
0.485 |
0.125 |
1.80E-12 |
LOC107446454 |
uncharacterized
LOC107446454 |
aug3.g19176 |
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| LOC107446115 |
3.52E-16 |
0.97844531 |
0.928 |
0.711 |
6.21E-12 |
LOC107446115 |
extracellular
sulfatase Sulf-1 |
aug3.g18939 |
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ptc5 - |
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fuchi5 + |
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| LOC107450380 |
3.88E-16 |
0.91516495 |
0.918 |
0.74 |
6.86E-12 |
LOC107450380 |
uncharacterized
protein y4hQ-like |
aug3.g3292 |
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| LOC107456525 |
3.90E-16 |
1.54367072 |
0.68 |
0.279 |
6.88E-12 |
LOC107456525 |
delta-like
protein 1 |
aug3.g25248 |
Pt-Delta |
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fuchi3 - |
fuchi5 - |
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| LOC107438301 |
4.90E-16 |
0.72988688 |
0.557 |
0.179 |
8.65E-12 |
LOC107438301 |
zinc finger
protein 236, transcript variant X1 |
aug3.g12961 |
Pt-zfp236 |
hh5 + |
ptc5 - |
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| LOC107453612 |
7.06E-16 |
0.67540907 |
0.66 |
0.213 |
1.25E-11 |
LOC107453612 |
protein
Wnt-8b-like, transcript variant X1 |
aug3.g5617 |
Pt-wnt8 |
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ptc5 - |
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fuchi5 - |
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| LOC107444253 |
1.84E-15 |
0.76136625 |
0.598 |
0.206 |
3.25E-11 |
LOC107444253 |
transcription
factor HES-1-A |
aug3.g17723 |
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ptc5 - |
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| LOC107447970 |
5.30E-14 |
0.62443423 |
1 |
0.887 |
9.36E-10 |
LOC107447970 |
aurora kinase
C-like |
aug3.g15230 |
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hh5 + |
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| LOC107446080 |
7.09E-13 |
0.84713974 |
0.732 |
0.392 |
1.25E-08 |
LOC107446080 |
cyclin-dependent
kinase 20 |
aug3.g1238 |
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hh5 + |
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| LOC107456960 |
1.29E-12 |
0.75792837 |
0.876 |
0.689 |
2.28E-08 |
LOC107456960 |
suppressor of
cytokine signaling 2, transcript variant X4 |
aug3.g25340 |
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hh5 - |
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| LOC107451332 |
2.95E-12 |
0.53215765 |
0.268 |
0.047 |
5.21E-08 |
LOC107451332 |
uncharacterized
LOC107451332 |
aug3.g3963 |
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ptc5 - |
msx6 + |
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| LOC107442050 |
1.19E-11 |
0.78587193 |
0.629 |
0.328 |
2.10E-07 |
LOC107442050 |
laminin subunit
alpha-1-like, transcript variant X2 |
aug3.g16317 |
Pt-laminin-alpha-1 |
hh5 - |
ptc5 - |
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| LOC107439672 |
1.36E-11 |
0.83737717 |
0.897 |
0.711 |
2.40E-07 |
LOC107439672 |
serine/threonine-protein
kinase pim-3 |
aug3.g13957 |
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hh5 - |
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| LOC107456506 |
8.12E-11 |
0.6104688 |
0.268 |
0.054 |
1.43E-06 |
LOC107456506 |
uncharacterized
LOC107456506, transcript variant X3 |
aug3.g8629 |
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hh5 + |
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| LOC107448343 |
8.46E-10 |
0.59791006 |
0.351 |
0.11 |
1.49E-05 |
LOC107448343 |
uncharacterized
LOC107448343, transcript variant X1 |
aug3.g15272 |
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fuchi5 - |
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| LOC107455285 |
1.22E-09 |
0.82462984 |
0.546 |
0.277 |
2.15E-05 |
LOC107455285 |
abhydrolase
domain-containing protein C22H12.03, transcript
variant X2 |
aug3.g6972 |
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ptc5 - |
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| LOC107450335 |
1.90E-09 |
0.59584094 |
0.32 |
0.093 |
3.35E-05 |
LOC107450335 |
zinc finger
protein basonuclin-2-like, transcript variant X1 |
aug3.g20127 |
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| LOC107451299 |
4.21E-09 |
0.30571167 |
1 |
1 |
7.43E-05 |
LOC107451299 |
tubulin alpha
chain, testis-specific-like |
aug3.g10572 |
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hh5 + |
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| LOC107444097 |
4.70E-09 |
0.50596131 |
0.979 |
0.956 |
8.30E-05 |
LOC107444097 |
kinesin-like
protein KIF20B |
aug3.g17615 |
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| LOC107449543 |
6.93E-09 |
0.4935582 |
1 |
0.9 |
1.22E-04 |
LOC107449543 |
ribonucleoside-diphosphate
reductase subunit M2-like |
aug3.g2820 |
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| LOC107447988 |
5.93E-08 |
0.50255519 |
0.443 |
0.199 |
1.05E-03 |
LOC107447988 |
homeobox protein
engrailed-like ceh-16 |
aug3.g15243 |
Pt-noto1 |
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ptc5 - |
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| LOC107440619 |
9.18E-08 |
0.58586783 |
0.876 |
0.73 |
1.62E-03 |
LOC107440619 |
ETS
domain-containing protein Elk-1, transcript variant X1 |
aug3.g9766 |
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| LOC107449038 |
3.15E-07 |
0.41950057 |
0.34 |
0.127 |
5.57E-03 |
LOC107449038 |
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| LOC107442180 |
3.31E-07 |
0.26558019 |
1 |
0.998 |
5.85E-03 |
LOC107442180 |
uncharacterized
protein DDB_G0286299-like |
aug3.g16410 |
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| LOC107443158 |
3.57E-07 |
0.4958024 |
0.928 |
0.792 |
6.30E-03 |
LOC107443158 |
uncharacterized
protein y4hQ-like |
aug3.g17068 |
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| LOC107438732 |
3.72E-07 |
0.66033446 |
0.742 |
0.52 |
6.57E-03 |
LOC107438732 |
somatomedin-B
and thrombospondin type-1 domain-containing
protein-like |
aug3.g26535 |
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ptc5 - |
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| LOC107457295 |
4.42E-07 |
0.43930517 |
0.938 |
0.897 |
7.82E-03 |
LOC107457295 |
fructose-bisphosphate
aldolase-like |
aug3.g11046 |
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| *pct1 and pct2
mean proportion of cells that express the gene in this cluster and other
clusters, respectively. |
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| **hh5, hh pRNAi at late stage 5;
ptc5. ptc
pRNAi at late stage 5; msx6, msx1 pRNAi at stage 6;
fuchi3, fuchi pRNAi at stage 3;
fuchi5, fuchi pRNAi at stage 5; +, expression increase; -, expression
decrease |
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