Table S7. Statistics of the second single-cell and the
single-nucleus RNA-seq experiment. |
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Genome |
Ver.2 |
Ver.2 |
Ver.3 |
Ver.3 |
Ver.3_modified |
Ver.3_modified |
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Library |
cell |
nuc |
cell |
nuc |
cell |
nuc |
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Total_Reads_in_FASTQ |
262,789,250 |
212,772,149 |
262,789,250 |
212,772,149 |
262,789,250 |
212,772,149 |
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Total_Filtered_Reads |
253,646,759 |
208,940,826 |
253,646,759 |
208,940,826 |
253,646,759 |
208,940,826 |
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Q30_Bases_in_Filtered_R2
(%) |
89.62 |
88.39 |
89.62 |
88.39 |
89.62 |
88.39 |
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Reads
Assigned_to_Cell_Labels (%) |
90.76 |
88.94 |
90.76 |
88.94 |
90.76 |
88.94 |
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Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome
(%) |
54.3 |
21.4 |
58.99 |
24.52 |
60.88 |
47.25 |
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Cellular_Reads_Aligned_Uniquely_to_Other_Genomic_Regions
(%) |
26.66 |
56.28 |
24.08 |
52.74 |
22.4 |
31.37 |
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Cellular_Reads_Aligned_Not_Unique
(%) |
4.04 |
5.26 |
2.34 |
5.26 |
2.22 |
3.9 |
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Cellular_Reads_Unaligned
(%) |
1.8 |
0.91 |
1.4 |
1.34 |
1.31 |
1.34 |
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Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome |
137,737,312 |
44,721,667 |
149,621,659 |
51,225,493 |
154,420,539 |
98,722,283 |
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Total_Raw_Molecules |
89,663,338 |
14,926,154 |
97,293,309 |
17,026,410 |
100,504,366 |
33,092,094 |
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Total_RSEC_Molecules |
88,525,400 |
14,117,753 |
96,057,558 |
16,098,977 |
99,241,542 |
31,363,366 |
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Mean_Raw_Sequencing_Depth |
1.54 |
3 |
1.54 |
3.01 |
1.54 |
2.98 |
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Mean_RSEC_Sequencing_Depth |
1.56 |
3.17 |
1.56 |
3.18 |
1.56 |
3.15 |
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Sequencing_Saturation |
58.97 |
89.9 |
59.07 |
90.02 |
58.96 |
89.62 |
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Putative_Cell_Count |
5,308 |
5,426 |
5,361 |
5,218 |
5,501 |
5,145 |
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Mean_Reads_per_Cell |
15914.78 |
7134.91 |
17078.24 |
8455.39 |
17343.24 |
16985.31 |
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Mean_Molecules_per_Cell |
10204.34 |
2234.35 |
10936.98 |
2637.34 |
11116.96 |
5362.98 |
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Mean_Targets_per_Cell |
2350.58 |
1353.8 |
2505.43 |
1528.25 |
2625.36 |
2482.56 |
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Total_Targets_Detected |
16,488 |
17,000 |
17,493 |
18,352 |
19,114 |
20,403 |
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