| Table S7. Statistics of the second single-cell and the single-nucleus RNA-seq experiment. | ||||||||||||||
| Genome | Ver.2 | Ver.2 | Ver.3 | Ver.3 | Ver.3_modified | Ver.3_modified | ||||||||
| Library | cell | nuc | cell | nuc | cell | nuc | ||||||||
| Total_Reads_in_FASTQ | 262,789,250 | 212,772,149 | 262,789,250 | 212,772,149 | 262,789,250 | 212,772,149 | ||||||||
| Total_Filtered_Reads | 253,646,759 | 208,940,826 | 253,646,759 | 208,940,826 | 253,646,759 | 208,940,826 | ||||||||
| Q30_Bases_in_Filtered_R2 (%) | 89.62 | 88.39 | 89.62 | 88.39 | 89.62 | 88.39 | ||||||||
| Reads Assigned_to_Cell_Labels (%) | 90.76 | 88.94 | 90.76 | 88.94 | 90.76 | 88.94 | ||||||||
| Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome (%) | 54.3 | 21.4 | 58.99 | 24.52 | 60.88 | 47.25 | ||||||||
| Cellular_Reads_Aligned_Uniquely_to_Other_Genomic_Regions (%) | 26.66 | 56.28 | 24.08 | 52.74 | 22.4 | 31.37 | ||||||||
| Cellular_Reads_Aligned_Not_Unique (%) | 4.04 | 5.26 | 2.34 | 5.26 | 2.22 | 3.9 | ||||||||
| Cellular_Reads_Unaligned (%) | 1.8 | 0.91 | 1.4 | 1.34 | 1.31 | 1.34 | ||||||||
| Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome | 137,737,312 | 44,721,667 | 149,621,659 | 51,225,493 | 154,420,539 | 98,722,283 | ||||||||
| Total_Raw_Molecules | 89,663,338 | 14,926,154 | 97,293,309 | 17,026,410 | 100,504,366 | 33,092,094 | ||||||||
| Total_RSEC_Molecules | 88,525,400 | 14,117,753 | 96,057,558 | 16,098,977 | 99,241,542 | 31,363,366 | ||||||||
| Mean_Raw_Sequencing_Depth | 1.54 | 3 | 1.54 | 3.01 | 1.54 | 2.98 | ||||||||
| Mean_RSEC_Sequencing_Depth | 1.56 | 3.17 | 1.56 | 3.18 | 1.56 | 3.15 | ||||||||
| Sequencing_Saturation | 58.97 | 89.9 | 59.07 | 90.02 | 58.96 | 89.62 | ||||||||
| Putative_Cell_Count | 5,308 | 5,426 | 5,361 | 5,218 | 5,501 | 5,145 | ||||||||
| Mean_Reads_per_Cell | 15914.78 | 7134.91 | 17078.24 | 8455.39 | 17343.24 | 16985.31 | ||||||||
| Mean_Molecules_per_Cell | 10204.34 | 2234.35 | 10936.98 | 2637.34 | 11116.96 | 5362.98 | ||||||||
| Mean_Targets_per_Cell | 2350.58 | 1353.8 | 2505.43 | 1528.25 | 2625.36 | 2482.56 | ||||||||
| Total_Targets_Detected | 16,488 | 17,000 | 17,493 | 18,352 | 19,114 | 20,403 | ||||||||