Table S7. Statistics of the second single-cell and the single-nucleus RNA-seq experiment.
Genome Ver.2 Ver.2 Ver.3 Ver.3 Ver.3_modified Ver.3_modified
Library cell nuc cell nuc cell nuc
Total_Reads_in_FASTQ 262,789,250 212,772,149 262,789,250 212,772,149 262,789,250 212,772,149
Total_Filtered_Reads 253,646,759 208,940,826 253,646,759 208,940,826 253,646,759 208,940,826
Q30_Bases_in_Filtered_R2 (%) 89.62 88.39 89.62 88.39 89.62 88.39
Reads Assigned_to_Cell_Labels (%) 90.76 88.94 90.76 88.94 90.76 88.94
Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome (%) 54.3 21.4 58.99 24.52 60.88 47.25
Cellular_Reads_Aligned_Uniquely_to_Other_Genomic_Regions (%) 26.66 56.28 24.08 52.74 22.4 31.37
Cellular_Reads_Aligned_Not_Unique (%) 4.04 5.26 2.34 5.26 2.22 3.9
Cellular_Reads_Unaligned (%) 1.8 0.91 1.4 1.34 1.31 1.34
Cellular_Reads_Aligned_Uniquely_to_Annotated_Transcriptome 137,737,312 44,721,667 149,621,659 51,225,493 154,420,539 98,722,283
Total_Raw_Molecules 89,663,338 14,926,154 97,293,309 17,026,410 100,504,366 33,092,094
Total_RSEC_Molecules 88,525,400 14,117,753 96,057,558 16,098,977 99,241,542 31,363,366
Mean_Raw_Sequencing_Depth 1.54 3 1.54 3.01 1.54 2.98
Mean_RSEC_Sequencing_Depth 1.56 3.17 1.56 3.18 1.56 3.15
Sequencing_Saturation 58.97 89.9 59.07 90.02 58.96 89.62
Putative_Cell_Count 5,308 5,426 5,361 5,218 5,501 5,145
Mean_Reads_per_Cell 15914.78 7134.91 17078.24 8455.39 17343.24 16985.31
Mean_Molecules_per_Cell 10204.34 2234.35 10936.98 2637.34 11116.96 5362.98
Mean_Targets_per_Cell 2350.58 1353.8 2505.43 1528.25 2625.36 2482.56
Total_Targets_Detected 16,488 17,000 17,493 18,352 19,114 20,403