| Table S9.1. Cluster 6 markers identified in the single-nucleus
RNA-seq analysis. |
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| Gene ID (NCBI) |
p_val |
avg_log2FC |
*pct.1 |
*pct.2 |
p_val_adj |
Gene ID (NCBI) |
Product (NCBI) |
aug3 gene model |
Gene name |
Expression in RNAi embryos** |
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| LOC107440074 |
0 |
3.10213806 |
0.777 |
0.057 |
0 |
LOC107440074 |
uncharacterized LOC107440074 |
aug3.g407 |
Pt-RTP-B |
hh5 + |
ptc5 - |
msx6 - |
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| LOC107456525 |
6.44E-200 |
2.44372473 |
0.91 |
0.249 |
1.31E-195 |
LOC107456525 |
delta-like protein 1 |
aug3.g25248 |
Pt-Delta |
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fuchi3 - |
fuchi5 - |
| LOC107442300 |
3.50E-158 |
0.68115914 |
0.186 |
0.002 |
7.12E-154 |
LOC107442300 |
flocculation protein FLO11 |
aug3.g16493 |
(homeodomain
protein) |
hh5 + |
ptc5 - |
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| LOC107436465 |
3.48E-118 |
1.22318986 |
0.591 |
0.115 |
7.08E-114 |
LOC107436465 |
B-cell lymphoma/leukemia 11A |
aug3.g26043 |
Pt-bcl11 |
hh5 + |
ptc5 - |
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| LOC107441660 |
4.68E-88 |
1.74263408 |
0.957 |
0.668 |
9.51E-84 |
LOC107441660 |
unconventional myosin-Ie |
aug3.g9845,aug3.g9846,aug3.g22123 |
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| LOC107452006 |
1.86E-86 |
1.40406924 |
0.904 |
0.402 |
3.77E-82 |
LOC107452006 |
transcription factor AP-2-beta |
aug3.g23531 |
Pt-AP2-A |
hh5 + |
ptc5 - |
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| LOC107447504 |
2.29E-84 |
1.311805 |
0.641 |
0.189 |
4.66E-80 |
LOC107447504 |
homeobox protein Hox-B5b |
aug3.g1787,aug3.g1786 |
Pt-ftz |
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ptc5 - |
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| LOC107446080 |
1.24E-69 |
1.42982066 |
0.867 |
0.505 |
2.52E-65 |
LOC107446080 |
cyclin-dependent kinase 20 |
aug3.g1238 |
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hh5 + |
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| LOC107442208 |
9.94E-69 |
1.45892116 |
0.693 |
0.269 |
2.02E-64 |
LOC107442208 |
protein shifted |
aug3.g27568 |
Pr-shifted |
hh5 + |
ptc5 - |
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| LOC107450899 |
4.81E-66 |
1.32779401 |
0.941 |
0.592 |
9.77E-62 |
LOC107450899 |
sal-like protein 1 |
aug3.g3675 |
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ptc5 - |
msx6 - |
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fuchi5 - |
| LOC107438015 |
5.08E-62 |
0.82855856 |
0.409 |
0.1 |
1.03E-57 |
LOC107438015 |
growth arrest-specific protein 1 |
aug3.g9575 |
Pt-gas1 |
hh5 + |
ptc5 - |
msx6 - |
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| LOC107443647 |
2.19E-56 |
1.04084339 |
0.854 |
0.472 |
4.45E-52 |
LOC107443647 |
plexin-B |
aug3.g17369 |
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fuchi5 - |
| LOC107445228 |
5.15E-53 |
0.92965127 |
0.65 |
0.241 |
1.05E-48 |
LOC107445228 |
protein gooseberry |
aug3.g18397 |
Pt-prd2
(pax3/7B) |
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ptc5 - |
msx6 - |
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| LOC107447180 |
1.08E-51 |
0.71829217 |
0.848 |
0.391 |
2.20E-47 |
LOC107447180 |
protein Wnt-5b-like |
aug3.g1632 |
Pt-wnt5 |
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ptc5 - |
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| LOC107447970 |
6.37E-47 |
0.89953393 |
0.957 |
0.891 |
1.29E-42 |
LOC107447970 |
aurora kinase C |
aug3.g15230 |
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hh5 + |
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| LOC107447678 |
9.22E-46 |
0.76870344 |
0.474 |
0.157 |
1.87E-41 |
LOC107447678 |
homeobox protein MSH-D |
aug3.g15189 |
Pt-msx1 |
hh5 + |
ptc5 - |
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| LOC107451685 |
2.94E-41 |
0.71537707 |
0.882 |
0.543 |
5.97E-37 |
LOC107451685 |
roundabout homolog 3 |
aug3.g10604 |
Pt-echinoid |
hh5 + |
ptc5 - |
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| LOC107453612 |
5.61E-38 |
0.57310652 |
0.638 |
0.274 |
1.14E-33 |
LOC107453612 |
protein Wnt-8b-like |
aug3.g5617 |
Pt-wnt8 |
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ptc5 - |
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fuchi5 - |
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| LOC107440707 |
7.75E-33 |
1.0401822 |
0.356 |
0.124 |
1.57E-28 |
LOC107440707 |
homeobox protein Mix.2-like |
aug3.g27124 |
Pt-zen |
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ptc5 - |
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| LOC107457387 |
1.39E-31 |
0.41962791 |
0.127 |
0.019 |
2.82E-27 |
LOC107457387 |
helix-loop-helix protein 13-like |
aug3.g3782 |
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hh5 + |
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| LOC107457368 |
3.46E-29 |
0.48585993 |
0.217 |
0.057 |
7.03E-25 |
LOC107457368 |
uncharacterized LOC107457368 |
aug3.g25891 |
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| LOC107448645 |
6.49E-29 |
0.65869826 |
0.814 |
0.5 |
1.32E-24 |
LOC107448645 |
transcription factor Sp9 |
aug3.g2333 |
Pt-sp6-9 |
hh5 - |
ptc5 - |
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| LOC107444558 |
3.86E-27 |
0.64845714 |
0.542 |
0.261 |
7.85E-23 |
LOC107444558 |
paired box protein Pax-5 |
aug3.g762 |
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ptc5 - |
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| LOC107456102 |
4.46E-27 |
0.31883712 |
0.152 |
0.031 |
9.07E-23 |
LOC107456102 |
uncharacterized LOC107456102 |
aug3.g8310 |
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| LOC107448079 |
4.98E-25 |
0.95404359 |
0.461 |
0.236 |
1.01E-20 |
LOC107448079 |
putative epidermal cell surface
receptor |
aug3.g20118 |
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| LOC107436894 |
1.68E-23 |
0.6555266 |
0.91 |
0.704 |
3.41E-19 |
LOC107436894 |
zinc finger protein rotund |
aug3.g394 |
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| LOC107443364 |
1.04E-21 |
0.55128228 |
0.82 |
0.563 |
2.11E-17 |
LOC107443364 |
uncharacterized LOC107443364 |
aug3.g17172 |
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hh5 + |
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| LOC107438714 |
5.29E-20 |
0.54923617 |
0.935 |
0.782 |
1.08E-15 |
LOC107438714 |
transcriptional activator cubitus
interruptus-like |
aug3.g13321 |
Pt-ci |
hh5 + |
ptc5 - |
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| LOC107446595 |
1.01E-19 |
0.51603907 |
0.446 |
0.222 |
2.06E-15 |
LOC107446595 |
uncharacterized LOC107446595 |
aug3.g1396 |
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ptc5 - |
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| LOC107446454 |
7.39E-19 |
0.6924459 |
0.195 |
0.064 |
1.50E-14 |
LOC107446454 |
uncharacterized LOC107446454 |
aug3.g19176 |
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| LOC107447507 |
4.90E-18 |
0.35996815 |
0.173 |
0.054 |
9.96E-14 |
LOC107447507 |
homeobox protein Hox-A5 |
aug3.g17640 |
Pt-scr-B |
hh5 + |
ptc5 - |
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| LOC107446220 |
9.47E-18 |
0.50909714 |
0.681 |
0.443 |
1.92E-13 |
LOC107446220 |
transcription factor Sox-14-like |
aug3.g24914,aug3.g24896 |
Pt-soxN |
hh5 + |
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| LOC107442927 |
5.79E-17 |
0.40095021 |
0.991 |
0.889 |
1.18E-12 |
LOC107442927 |
uncharacterized LOC107442927 |
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| LOC107451091 |
1.31E-15 |
0.537012 |
0.173 |
0.06 |
2.67E-11 |
LOC107451091 |
uncharacterized LOC107451091 |
aug3.g3782,aug3.g11118 |
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hh5 + |
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| LOC107449746 |
2.98E-15 |
0.63519606 |
0.322 |
0.156 |
6.06E-11 |
LOC107449746 |
nuclear receptor subfamily 2
group F member 1-A |
aug3.g2915 |
Pt-seven-up-A |
hh5 + |
ptc5 + |
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| LOC107448451 |
5.04E-15 |
0.39996009 |
0.22 |
0.088 |
1.02E-10 |
LOC107448451 |
band 4.1-like protein 4 |
aug3.g2228 |
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ptc5 - |
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| LOC107455776 |
2.55E-14 |
0.28583497 |
0.198 |
0.076 |
5.18E-10 |
LOC107455776 |
DEAD-box helicase Dbp80 |
aug3.g8013 |
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| LOC122272790 |
3.15E-14 |
0.44028205 |
0.307 |
0.153 |
6.40E-10 |
LOC122272790 |
Werner syndrome ATP-dependent
helicase homolog |
aug3.g11632 |
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| LOC107447768 |
8.35E-14 |
0.67972787 |
0.189 |
0.076 |
1.70E-09 |
LOC107447768 |
CRISP/Allergen/PR-1 |
aug3.g1919 |
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| LOC107454244 |
1.08E-13 |
0.34042525 |
0.991 |
0.952 |
2.20E-09 |
LOC107454244 |
axin-1 |
aug3.g6117 |
Pt-axin |
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| LOC107450670 |
3.80E-13 |
0.2685105 |
0.139 |
0.045 |
7.73E-09 |
LOC107450670 |
uncharacterized LOC107450670 |
aug3.g15599 |
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ptc5 - |
msx6 - |
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| LOC107442485 |
1.66E-12 |
0.48204534 |
0.706 |
0.525 |
3.38E-08 |
LOC107442485 |
partitioning defective 3 homolog |
aug3.g27669 |
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| LOC107442050 |
4.41E-12 |
0.47518093 |
0.783 |
0.632 |
8.95E-08 |
LOC107442050 |
laminin subunit alpha-1 |
aug3.g16317 |
Pt-laminin-alpha-1 |
hh5 - |
ptc5 - |
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| LOC107436691 |
8.12E-12 |
0.41356645 |
0.889 |
0.704 |
1.65E-07 |
LOC107436691 |
uncharacterized LOC107436691 |
aug3.g11765 |
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hh5 - |
ptc5 - |
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| LOC107457295 |
1.06E-11 |
0.26603576 |
0.585 |
0.388 |
2.15E-07 |
LOC107457295 |
fructose-bisphosphate aldolase |
aug3.g11046 |
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| LOC107438320 |
1.53E-11 |
0.38245677 |
0.173 |
0.071 |
3.11E-07 |
LOC107438320 |
uncharacterized LOC107438320 |
aug3.g12989 |
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hh5 + |
ptc5 - |
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| LOC107450033 |
1.81E-10 |
0.41238712 |
0.678 |
0.518 |
3.68E-06 |
LOC107450033 |
protein pangolin, isoforms
A/H/I/S |
aug3.g10490,aug3.g18357 |
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| LOC107448951 |
2.75E-10 |
0.49015494 |
0.186 |
0.085 |
5.59E-06 |
LOC107448951 |
BTB/POZ domain-containing protein
kctd15-like |
aug3.g2480 |
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hh5 + |
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fuchi5 + |
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| LOC107450335 |
5.19E-09 |
0.38799842 |
0.44 |
0.292 |
0.00010539 |
LOC107450335 |
zinc finger protein basonuclin-2 |
aug3.g3273 |
Pt-basonuclin-A |
hh5 + |
ptc5 - |
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| LOC107446878 |
5.37E-09 |
0.27770492 |
0.223 |
0.115 |
0.00010905 |
LOC107446878 |
AT-rich interactive
domain-containing protein 5B |
aug3.g1504 |
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hh5 + |
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| LOC107448725 |
7.58E-09 |
0.25365884 |
0.991 |
0.97 |
0.00015395 |
LOC107448725 |
histone-lysine
N-methyltransferase trithorax |
aug3.g2380,aug3.g2379 |
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| LOC107440619 |
7.81E-09 |
0.3691937 |
0.864 |
0.763 |
0.00015872 |
LOC107440619 |
ETS domain-containing protein
Elk-1 |
aug3.g9766 |
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| LOC107454259 |
1.05E-08 |
0.25311205 |
0.266 |
0.147 |
0.00021238 |
LOC107454259 |
protocadherin-like wing polarity
protein stan |
aug3.g15160,aug3.g6142,aug3.g22281 |
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ptc5 - |
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| LOC107445694 |
1.30E-08 |
0.29512656 |
0.954 |
0.835 |
0.00026482 |
LOC107445694 |
unconventional myosin-Vb |
aug3.g9254,aug3.g6148,aug3.g21618 |
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| LOC122271496 |
1.67E-08 |
0.28666169 |
0.152 |
0.068 |
0.00033866 |
LOC122271496 |
uncharacterized LOC122271496 |
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| LOC107452024 |
2.81E-08 |
0.41352158 |
0.176 |
0.087 |
0.00057007 |
LOC107452024 |
uncharacterized LOC107452024 |
aug3.g4456 |
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| LOC107456104 |
3.53E-08 |
0.37053016 |
0.341 |
0.209 |
0.00071664 |
LOC107456104 |
uncharacterized LOC107456104 |
aug3.g10934,aug3.g18321 |
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ptc5 - |
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| *pct1 and pct2
mean proportion of cells that express the gene in this cluster and other
clusters, respectively. |
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| **hh5, hh pRNAi at late stage 5;
ptc5. ptc
pRNAi at late stage 5; msx6, msx1 pRNAi at stage 6;
fuchi3, fuchi pRNAi at stage 3;
fuchi5, fuchi pRNAi at stage 5; +, expression increase; -, expression
decrease |
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