Table S4.2. Cluster 2 markers  identified in the first single-cell RNA-seq analysis.
Gene ID (NCBI) p_val avg_log2FC *pct.1 *pct.2 p_val_adj Gene ID (NCBI) Product (NCBI) aug3 gene model Gene name **Expression in RNAi embryos    
LOC107451809 1.31E-47 2.78767291 0.913 0.111 2.31E-43 LOC107451809 sonic hedgehog protein-like aug3.g4322 Pt-hh hh5 -     fuchi3 -  
LOC107438641 5.30E-38 1.96252242 0.891 0.142 9.37E-34 LOC107438641 transcription factor Sox-13, transcript variant X5 aug3.g13250            
LOC107457189 5.98E-36 2.42653968 1 0.244 1.06E-31 LOC107457189 homeobox protein OTX2-A-like aug3.g9172 Pt-otd (oc2) hh5 - ptc5 +      
LOC107451908 2.36E-27 1.49926982 0.478 0.039 4.16E-23 LOC107451908 uncharacterized LOC107451908 aug3.g4396   hh5 - ptc5 +      
LOC107444665 6.85E-21 0.9864488 0.587 0.094 1.21E-16 LOC107444665 nuclear receptor subfamily 2 group E member 1-like aug3.g794 Pt-tll hh5 -       fuchi5 -
LOC107438011 1.15E-15 1.50019269 0.804 0.301 2.03E-11 LOC107438011 neurogenic locus protein delta-like, transcript variant X1 aug3.g12734 Pt-Delta2 hh5 - ptc5 +     fuchi5 -
LOC107456634 2.81E-14 0.82706573 1 0.987 4.96E-10 LOC107456634 RNA polymerase-associated protein CTR9 homolog aug3.g8712            
LOC107453313 1.11E-13 1.24459289 0.87 0.449 1.95E-09 LOC107453313 protein naked cuticle homolog 1 aug3.g5391 Pt-naked cuticle hh5 - ptc5 +      
LOC107453612 2.31E-13 1.76761156 0.696 0.259 4.08E-09 LOC107453612 protein Wnt-8b-like, transcript variant X1 aug3.g5617 Pt-wnt8   ptc5 -     fuchi5 -
LOC107452215 9.55E-13 1.337603 0.717 0.298 1.69E-08 LOC107452215 Krueppel-like factor 7, transcript variant X1 aug3.g4601   hh5 -        
LOC107444744 1.02E-12 0.50724998 0.261 0.026 1.80E-08 LOC107444744 transcription factor SOX-8 aug3.g816   hh5 -   msx6 -    
LOC107457313 1.46E-12 1.65698365 0.674 0.27 2.58E-08 LOC107457313 transcription factor Sox-2 aug3.g11061 Pt-sox2-A hh5 -   msx6 -    
LOC107440157 4.34E-11 0.97199291 0.804 0.359 7.66E-07 LOC107440157 zinc finger protein 423-like, transcript variant X3 aug3.g14296 Pt-znf423 hh5 - ptc5 +      
LOC107457180 2.03E-10 0.93683284 0.761 0.351 3.58E-06 LOC107457180 protein jagged-1-like aug3.g9161   hh5 -        
LOC107439618 3.23E-10 0.7224142 0.565 0.198 5.70E-06 LOC107439618 uncharacterized LOC107439618, transcript variant X2 aug3.g13925   hh5 -        
LOC107437681 1.17E-09 0.59689093 0.587 0.174 2.06E-05 LOC107437681 integrin alpha-8-like aug3.g7077   hh5 -        
LOC107456630 4.90E-09 0.33186794 0.283 0.05 8.65E-05 LOC107456630 intracellular protein transport protein USO1-like aug3.g8711           fuchi5 -
LOC107454763 1.68E-08 0.34550355 0.261 0.046 2.97E-04 LOC107454763 F-box/LRR-repeat protein 14-like aug3.g10819            
LOC107455300 2.14E-08 0.63599271 0.565 0.19 3.77E-04 LOC107455300 silk gland factor 1-like aug3.g6985 Pt-fkh hh5 -       fuchi5 -
LOC107438758 8.31E-08 0.6729294 0.87 0.556 1.47E-03 LOC107438758 epidermal growth factor receptor, transcript variant X2 aug3.g13345            
LOC107436800 3.83E-07 0.80786841 0.565 0.257 6.77E-03 LOC107436800 uncharacterized LOC107436800 aug3.g11835           fuchi5 +
*pct1 and pct2 mean proportion of cells that express the gene in this cluster and other clusters, respectively.
**hh5, hh pRNAi at late stage 5; ptc5. ptc pRNAi at late stage 5; msx6, msx1 pRNAi at stage 6;  fuchi3, fuchi pRNAi at stage 3;  fuchi5, fuchi pRNAi at stage 5; +, expression increase; -, expression decrease