| Table
S10. Clones |
|
|
|
|
|
|
|
|
| Genes identified
in the single-cell and single-nuc analyses |
|
|
|
|
|
|
|
| Gene ID (NCBI) |
Product (NCBI) |
aug3 gene model |
Gene name |
Isolation |
cDNA clone |
Primer 1 |
Primer 2 |
Accession number |
| LOC107447180 |
protein
Wnt-5b-like, transcript variant X1 |
aug3.g10820 |
Pt-wnt5 |
cDNA library
screening |
pBS-km-wnt5-#7-4-RV-PST#1 |
|
|
AB167810 |
| LOC107451660 |
vitamin
K-dependent protein C-like |
aug3.g4198 |
|
EST |
S7_d1_23_B05 |
|
|
|
| LOC107444844 |
protein
giant-lens-like |
aug3.g839 |
|
EST |
eS6_d1_50_G06 |
|
|
|
| LOC107450899 |
sal-like protein
1 |
aug3.g3675 |
|
PCR |
pCRII-LOC107450899#12 |
ggcacaacatgccgagtttg |
agttcggtggacgaggaaca |
|
| LOC107451065 |
uncharacterized
LOC107451065 |
aug3.g3755 |
|
EST |
eS7_SB_021_C08 |
|
|
|
| LOC107447504 |
homeobox protein
Hox-B4a-like, transcript variant X1 |
aug3.g1787 |
Pt-ftz |
EST |
eS6_d1_34_D05 |
|
|
LC379616 |
| LOC107450100 |
homeobox protein
DLX-6-like, transcript variant X1 |
aug3.g15531 |
Pt-dll |
PCR |
pBS-dll-DKS+3UTR-EI#3 |
gataaatctcaactggaagaaac |
aaatcaacttacccacccttc |
|
| LOC107444558 |
paired box
protein Pax-5, transcript variant X1 |
aug3.g762 |
|
EST |
At_eW_013_A08 |
|
|
|
| LOC107450101 |
dendritic arbor
reduction protein 1, transcript variant X1 |
aug3.g15550 |
|
EST |
At_eW_017_H24 |
|
|
|
| LOC107438641 |
transcription
factor Sox-13, transcript variant X5 |
aug3.g13250 |
|
EST |
S7_d1_19_F03 |
|
|
|
| LOC107444665 |
nuclear receptor
subfamily 2 group E member 1-like |
aug3.g794 |
Pt-tll |
5'RACE |
pCRII-km-tll-5RACE#1 |
gtctgcagtgcctgcactgatttctg |
|
LC379639 |
| LOC107452215 |
Krueppel-like
factor 7, transcript variant X1 |
aug3.g4601 |
|
EST |
At_eW_017_B16 |
|
|
|
| LOC107439896 |
gamma-butyrobetaine
dioxygenase |
aug3.g26914 |
|
Iwasaki-Yokozawa et al.
(2022) |
eS6_d1_21_D01 |
|
|
|
| LOC107449287 |
transcription
factor Sox-14-like |
aug3.g2665 |
|
EST |
eS6_d1_76_E04 |
|
|
|
| LOC107451770 |
uncharacterized
LOC107451770, transcript variant X1 |
aug3.g4286 |
|
EST |
eS6_d1_59_E12 |
|
|
|
| LOC107437681 |
integrin
alpha-8-like |
aug3.g7077 |
|
EST |
S7_d1_82_B12 |
|
|
|
| LOC107450281 |
group XV
phospholipase A2 |
aug3.g15560 |
|
EST |
eS6_d1_36_H01 |
|
|
|
| LOC107436092 |
LIM domain only
protein 3, transcript variant X3 |
aug3.g11301 |
|
PCR |
pCRII-LOC107436092#9 |
tacgcagaggccagcaatg |
gaagcaaactactaccgtcc |
|
| LOC107437526 |
fibroblast growth
factor receptor 3-like, transcript variant X1 |
aug3.g26271 |
Pt-FGFRa |
5'RACE |
pCRII-kmFGFR-A-5RACE#1 |
gccgctatgtcccgatgaatacagttc |
|
LC379614 |
| LOC107438841 |
fibroblast growth
factor receptor 4 |
aug3.g26565 |
Pt-FGFRb |
5'RACE |
pCRII-kmFGFR-B-5RACE#3 |
ctagcagccaagtctcgatgaatgcac |
|
LC379615 |
| LOC107453376 |
E3
ubiquitin-protein ligase PDZRN3 |
aug3.g23798 |
|
PCR |
pCRII-LOC107453376-ESP-QWF#3 |
gggaatcaccacctatagga |
acgacacctgaaccactgct |
|
| LOC107446932 |
8.6 kDa
transglutaminase substrate-like |
aug3.g1540 |
|
PCR |
pCRII-LOC107446932-LAT-DDN#2 |
cttagccacgatcgttcttc |
ttgtcatcagggacttctga |
|
| LOC107443223 |
slit homolog 1
protein-like |
aug3.g17108 |
|
PCR |
pCRII-LOC107443223-CAR-TSG#3 |
ctgcgcaagaaccttcaaca |
gttccactggtaatccaagg |
|
| LOC107451437 |
E3
ubiquitin-protein ligase ATL59-like |
aug3.g22384 |
|
PCR |
pCRII-LOC107451437-445F-827R#4 |
tggtcaggcaagtttcag |
tgcagactggacatgtag |
|
| LOC107448655 |
BMP-binding
endothelial regulator protein-like |
aug3.g2323 |
Pt-crossveinless2 |
PCR |
pCRII-LOC107448655-PRS-CSR#2 |
tcctacatcgtttggcagtg |
gggcacgactttctactgca |
|
| LOC107440592 |
uncharacterized
LOC107440592 |
aug3.g27105 |
|
PCR |
pCRII-LOC107440592-437F-1092R#3 |
tgccaagaaccgaaagca |
tggcacaggaataggaag |
|
| LOC107443723 |
proclotting
enzyme |
aug3.g17419 |
|
PCR |
pCRII-LOC107443723-TTM-TRN#1 |
actacgatgagtcctcgtca |
agtttctggtcaaccagtcc |
|
| LOC107448266 |
tyrosine-protein
phosphatase 10D-like |
aug3.g2169 |
|
EST |
At_eW_007_J03 |
|
|
|
| LOC107440570 |
uncharacterized
LOC107440570 |
aug3.g10457 |
|
EST |
eS7_SB_014_B10 |
|
|
|
| LOC107443591 |
BMP and activin
membrane-bound inhibitor homolog |
aug3.g17346 |
Pt-BAMBI1 |
EST |
At_eW_002_J14 |
|
|
LC379647 |
| LOC107453548 |
mothers against
decapentaplegic homolog 6, transcript variant X1 |
aug3.g5567 |
Pt-smad6 |
PCR |
pCRII-kmDad17-5 |
cgtgcggttctgtcaactgtg |
caacctttggcgaagctgatg |
LC379612 |
| LOC107447499 |
uncharacterized
LOC107447499 |
|
|
EST |
eS6_d1_47_E10 |
|
|
|
| LOC107450670 |
uncharacterized
LOC107450670 |
aug3.g15599 |
|
PCR |
pCRII-LOC107450670-1844F-2373R#5 |
actcttgcaggaatttcgag |
tcgccctttcaaatgctaac |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Other genes |
|
|
|
|
|
|
|
|
| Gene ID (NCBI) |
Product (NCBI) |
aug3 gene model |
Gene name |
Isolation |
cDNA clone |
Accession number |
|
|
| LOC107451427 |
glycerophosphocholine
phosphodiesterase GPCPD1, transcript variant X2 |
aug3.g15726 |
|
Akiyama-Oda & Oda (2010) |
At_eW_012_A08 |
AB433906 |
|
|
| LOC107440133 |
twist-related
protein 2-like |
aug3.g14287 |
Pt-twi |
Yamazaki et al. (2005) |
pBS-km-twi#6-1HIII#2 |
AB167807 |
|
|
| LOC107447678 |
homeobox protein
MSH-D-like |
aug3.g15189 |
Pt-msx1 |
Akiyama-Oda & Oda (2020) |
At_eW_020_B15 |
FY222578 |
|
|
| LOC107438948 |
rhomboid-related
protein 2 |
aug3.g13482 |
Pt-rhomboid |
Akiyama-Oda & Oda (2020) |
pBS-rhomboid |
LC379651 |
|
|
| LOC107456035 |
homeobox protein
B-H1 |
aug3.g8250 |
Pt-BarH1 |
Akiyama-Oda & Oda (2020) |
pCRII-aug3.g8250(f1-r1)#5 |
|
|
|
| LOC107451809 |
sonic hedgehog
protein-like |
aug3.g4322 |
Pt-hedgehog (hh) |
Oda et al. (2007) |
pZL-km.hh#10-2 |
AB125742 |
|
|
| LOC107456525 |
delta-like
protein 1 |
aug3.g25248 |
Pt-Delta |
Oda et al. (2007) |
pZL-km.Delta-dT#10-1 |
AB287420 |
|
|
| LOC107457189 |
homeobox protein
OTX2-A-like |
aug3.g9172 |
Pt-otd (oc2) |
Akiyama-Oda & Oda (2003) |
pZL-km.otd#8-1 |
AB096074 |
|
|
| LOC107437305 |
zinc finger
protein ZIC 4-like |
aug3.g12202 |
Pt-opa |
Kanayama et al. (2011) |
At_eW_022_A24 |
AB605264 |
|
|
| LOC107451717 |
SAM pointed
domain-containing Ets transcription factor-like |
aug3.g4238 |
Pt-Ets4 |
Iwasaki-Yokozawa et al.
(2022) |
aug3.g4238_F1R1_pBS_EcoRI |
|
|
|
| LOC107444265 |
noggin-2-like |
aug3.g17729 |
Pt-noggin-D |
Akiyama-Oda & Oda (2020) |
pCRII-aug3.g17729(f1-r1)#2 |
|
|
|
| LOC107448880 |
endothelial
transcription factor GATA-2-like, transcript
variant X3 |
aug3.g20514 |
Pt-GATA1 (gataC) |
Akiyama-Oda & Oda (2010) |
pCRII-kmGATA-C-3RACE#1 |
AB433902 |
|
|
| LOC107440074 |
uncharacterized
LOC107440074 |
aug3.g407 |
Pt-RTP-B |
Akiyama-Oda & Oda (2020) |
pCRII-aug3.g22741-5n(r2)#2 |
|
|
|
| LOC107442300 |
flocculation
protein FLO11 |
aug3.g16493 |
(homeodomain protein) |
Akiyama-Oda & Oda (2020) |
pCRII-aug3.g16493(f1-r1)#2 |
|
|
|
| LOC107452883 |
achaete-scute
homolog 1 |
aug3.g5047 |
Pt-asl-H |
Akiyama-Oda & Oda (2020) |
eS6_d1_32_G02 |
|
|
|
| LOC107453167 |
zinc finger
protein 37 |
aug3.g9318 |
Pt-krüppel-1 (krü-1) |
Akiyama-Oda & Oda (2020) |
S7_d1_70_G12 |
|
|
|
| LOC107442497 |
uncharacterized
LOC107442497 |
aug3.g27670,aug3.g20943 |
|
Akiyama-Oda & Oda (2020) |
pCRII-aug3.g9318(f1,r1)#2 |
|
|
|
|
|
|
|
|
|
|
|
|